There is a great variety of ncRNAs with a versatile function in prokaryotes and eukaryotes, but this chapter will focus on those ncRNAs that play a direct or an indirect role in the maintenance of genome stability and the regulation of DNA repair. In prokaryotes, ncRNAs are involved in the ...
This editorial discusses what levels of off-target effects can be tolerated in genome editing, in the context of various types of applications.
An ideal gene-editing technology should be able to transform a target DNA sequence into any other desired sequence while achieving high on-target editing rates (efficiency), and minimal off-target edits (specificity).7The life sciences have long aspired to create gene-editing tools that possess e...
This Collection provides an overview of current progress in developing targeted genome editing technologies, including a selection of protocols for using and adapting these tools in your own lab.
genome editing has already been promoted for a wide range of plant species. Although the application of genome-editing induces less unintended modifications (off-targets) in the genome compared to classical mutagenesis techniques, off-target effects are a prominent point of criticism as they are ...
The cytotoxic ribonuclease , off resonance rotating frame relaxation rates RNHNOE of the backbone amide N nuclei at two different magnetic field strengths (11.7 and 17.6 T). The two sets of relaxation parameters have been analysed in ter... José,Manuel,Pérez-Caadillas,... - 《Journal of...
Mutation identification can be assessed by several methods based on your experimental needs. For non-specific determination of the presence of a change in a genomic sequence,gel-based methodscan be used. For determining the success of on-target editing and for investigating off-target effects,next...
Unlike applications that aim primarily to alter DNA sequences, the effects of genome editing on gene regulation act at the transcript level, and could be used to reveal the function of many non-canonical RNAs that are related to crop improvement. As most non-coding transcripts are nuclear and...
For example, potential therapeutic applications of CRISPR/Cas9, which require specific editing in target tissues, might be challenged by exacerbating off-target effects caused by prolonged Cas9 activity.23 Moreover, Cas9 activity increases background mutagenesis in cases where it requires HDR-mediated ...
Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease. Nat Biotechnol. 2014;32:677–83. Article CAS PubMed Google Scholar Wang C, Shen L, Fu Y, Yan C, Wang K. A Simple CRISPR/Cas9 system for multiplex genome editing in rice. J Genet Genomics....