Enhanced homologous recombination by the modulation of targeting vector endsGenetic engineeringGenetic engineeringGenetic engineeringGenetic engineeringThe field of genome editing was founded on the establishment of methods, such as the clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR...
SBI’s HR Targeting Vectors are all that and more, with features that can make it easier to find successful clones, minimize the amount of vector DNA left in the genome, minimize off-target integration, and more. Need help choosing? Take a look at our table below, or contact technical ...
Targeted genome editing using engineered nucleases has rapidly gone from being a niche technology to a mainstream method used by many biological researchers. This widespread adoption has been largely fueled by the emergence of the clustered, regularly in
Zinc-finger nuclease-mediated gene correction using single AAV vector transduction and enhancement by Food and Drug Administration-approved drugs. An emerging strategy for the treatment of monogenic diseases uses genetic engineering to precisely correct the mutation(s) at the genome level. Recent adva....
Efficient transfer of base changes from a vector to the rice genome by homologous recombination: involvement of heteroduplex formation and mismatch correct... Through a gene-targeting procedure with positive-negative selection, we previously reported the generation of fertile transgenic rice plants with...
Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells Nat. Biotechnol., 33 (2015), pp. 985-989 CrossrefView in ScopusGoogle Scholar 96 I. Rivière, K. Brose, R.C. Mulligan Effects of retroviral vector design on expression of human adenosine deaminase in muri...
rAd has been continuously modified to enhance gene capacity, infection efficiency, duration of gene transduction and safety. The first-generation rAd lacks E1 and E3 regions and thus is replication incompetent and innate-immunity attenuated. The Ad5 vector from the first generation is predominantly us...
First, we constructed Cas9 expression vectors for in vitro transcription of Cas9 mRNA by subcloning a DNA fragment harboring the SP6 promoter sequence into a vector containing the nuclear-targeted humanized Cas9 coding sequence5. We also constructed a fusion of the crRNA...
However, several technical and scientific issues remain before these proof-of-principle demonstrations are advanced to effect vector population suppression. The development of a Y-drive has so far proven difficult because of the complete transcriptional shut down of the sex chromosomes during meiosis, ...
The vector containing Nat10tm1a was electroporated into C57BL/6 N derived JM8A3.N1 ES cells. Correct ES cell gene targeting was confirmed by long-range PCR and quantitative PCR. Targeted ES cells were microinjected into blastocysts and used to generate chimeras. Germ-line transmission was...