Genome editing in maize directed by CRISPR-Cas9 ribonucleoprotein complexes Nat. Commun., 7 (2016), p. 13274, 10.1038/ncomms13274 View in ScopusGoogle Scholar Tang et al., 2016 F. Tang, S.M. Yang, J.G. Liu, H.Y. Zhu Rj4, a gene controlling nodulation specificity in soybeans, encod...
Application of CRISPR-Cas12a temperature sensitivity for improved genome editing in rice, maize, and Arabidopsis. BMC Biol , 2019 , 17: 9 CrossRef PubMed Google Scholar [49] Endo A, Masafumi M, Kaya H, et al. Efficient targeted mutagenesis of rice and tobacco genomes using Cpf1 from...
To further confirm whether this graft-mobile gene editing system also works on other gene targets and thus in the context of other gRNA targeting sequences, we created two gRNAs, gVenus1and gVenus2, with and without fused TLS1 or TLS2 sequences. These gRNAs target an artificial35Spromoter:...
Genome Editing and Double-Fluorescence Proteins Enable Robust Maternal Haploid Induction and Identification in Maize Le Dong,Lina Li,Changlin Liu,etc.Genome Editing and Double-Fluorescence Proteins Enable Robust Maternal Haploid Induction and Identification in Maize[J].分子......
This Collection provides an overview of current progress in developing targeted genome editing technologies, including a selection of protocols for using and adapting these tools in your own lab.
We have developed a visualization toolbox, ViMeBox (VIsual Maize Editing toolBox), for the selection of positive transformants in maize (Zea mays). In the ViMeBox system, the vector expressing Cas9 includes a gene cassette containing a visible marker expressed from a tissue﹕pecific promoter; ...
Genome editing in maize directed by CRISPR-Cas9 ribonucleoprotein complexes Targeted DNA double-strand breaks have been shown to significantly increase the frequency and precision of genome editing. In the past two decades, several... Svitashev,Schwartz,Lenderts,... - 《Nature Communications》 被引...
CRISPR-Cas-Based Genome Editing for Crop Improvement: Progress, Challenges and Future Prospects It explores the applications of CRISPR-Cas-based genome editing in different crops, including maize, rice, wheat, and tomato, highlighting the progress ... G Ntsomboh-Ntsefong,E Seyum,TM Kingsley,.....
It is clear that the Breeding 4.0 era for maize will feature the integration of multi-disciplinary technologies including genomics and phenomics, gene editing and synthetic biology, and Big Data and artificial intelligence. The breeding approach of passively selecting ideal genotypes from designated ...
Genome editing by TALENs has been demonstrated in a wide variety of plants including Arabidopsis, Nicotiana, Brachypodium, barley, potato, tomato, sugarcane, flax, rapeseed, soybean, rice, maize, and wheat (reviewed in [12, 13]). The first application of TALEN-mediated genome editing in crop ...