The understanding of this biological phenomenon leads to the development of genome editing tool for making specific modifications in the genomes of various organisms. The CRISPR/Cas9 technology basically depends upon two components: Cas9 enzyme and sgRNA (single-guide RNA). These two components are ...
Horticultural crops provide humans with many valuable products. The improvement of the yield and quality of horticultural crops has been receiving increasing research attention. Given the development and advantages of genome-editing technologies, researc
Successful genome editing has been reported in a number of plants including Arabidopsis, rice, tobacco, apple, potato, maize and in various other crops shows the CRISPRs is an effective system for crop improvement. Improving quality is one of the key traits that determine the monetary worth of ...
Genome Engineering for Crop Improvement provides an up-to-date view of the use of genome editing for crop bio-fortification, improved bioavailability of minerals and nutrients, and enhanced hypo-allergenicity and hypo-immunogenicity. This volume examines a diversity of important topics including mineral...
The RNA editing-associated PLS-type PPR proteins feature additional C-terminal extensions: the E1 motif, the E2 motif, and the DYW domain, named for its distinctive Asp–Tyr–Trp C terminus (Cheng et al., 2016; Lurin et al., 2004; Figure 2A). The DYW domain harbors a sequence similar...
Widespread enthusiasm about potential contributions of genome-edited crops to address climate change, food security, nutrition and health, environmental sustainability and diversification of agriculture is dampened by concerns about the associated risks.
CRISPR/Cas9 genome editing has been widely adopted in plants as a tool for understanding fundamental biological processes (Belhaj et al., 2015, Voytas and Gao, 2014). Attention is now shifting toward the promise of genome editing for agricultural applications. In this study, we have taken a ma...
Coding sequence editing for trait improvement Increasing the crop yield is the primary purpose of crop breeding. The knockout of functional genes, such asGn1a,DEP1,GS3, andIPA1, which negatively regulate yield parameters such as panicle architecture, grain number, grain size, and plant architectu...
briefly overviewed the structure and mechanism of genome editing tools and then emphatically reviewed the advances in the application of genome editing tools for crop improvement, including the most recent case studies with CRISPR/Cpf1 and base-editing technologies. We have also discussed the future ...
The sequencing of large and complex genomes of crop species, facilitated by new sequencing technologies and bioinformatic approaches, has provided new opportunities for crop improvement. Current challenges include understanding how genetic variation tran