This, in turn, underscores its immense potential for crop improvement. Against this backdrop, the current Special Issue on "Genome Editing in Plants: A Tool for Precision Breeding and Functional Genomics" represents a timely effort to assemble a group of leading experts in the field of plant ...
plants in order to maximize benefits. The implementation of comprehensive synthetic biology tools, which are popularly known as “genome editing tools” [2], is required to carry out the task of integrating desired traits into crop genomes. Synthetic biology uses the rational design of biological...
Genome editing holds the potential for rapid crop improvement to meet the challenge of feeding the planet in a changing climate. The delivery of gene editing reagents into the plant cells has been dominated by plasmid vectors delivered using agrobacterium or particle bombardment. This approach involves...
low transcript abundance or lack ofCas9-TLS translation in distal tissues might result in insufficient or no editing activity. Therefore, we asked whether gene deletion edits are detectable in root and shoot tissue samples of grafted plants. A chloroticnia1mutant phenotype was detected in some leav...
genome editing during the fertilization stage, and the editing activity continued after the division of fertilized eggs. Regardless, the high frequency ofArabidopsisT1 transgenic plants showing the expected mutant phenotypes suggests that some of the mutations must have been generated very early in ...
Genome editing tools have been deployed in various model and non-model plants for functional characterization of genes and improvement of agricultural traits. This comprises the generation of plants with compositional characteristics and traits that improve quality and productivity of crops as well as ...
More recently, TALEN was shown to induce a variety of heritable mutations in rice [47], demonstrating its usefulness in plant genome editing. Table 2 TALEN mediated genome editing in plants Full size table As an effective genome editing tool, TALEN has been applied to generate useful traits in...
High-efficiency and multiplex adenine base editing in plants using new TadA variants 2021, Molecular Plant Citation Excerpt : These data, combined, indicate that TadA9 is highly active and well suited for use in multiplex gene editing in rice molecular breeding. Very recently other groups have rep...
We developed a CRISPR/Cas9 binary vector set based on the pGreen or pCAMBIA backbone, as well as a gRNA (guide RNA) module vector set, as a toolkit for multiplex genome editing in plants. This toolkit requires no restriction enzymes besides BsaI to generate final constructs harboring maize-co...
Site-specific genome editing in plants is a rapidly evolving field with tangible results. The technology is equipped with a powerful toolbox of molecular scissors to cut DNA at a pre-determined site with different efficiencies for designing an approach that best suits the objectives of each plant...