www.nature.com/npjsba ARTICLE OPEN IκBα is required for full transcriptional induction of some NFκB-regulated genes in response to TNF in MCF-7 cells Minami Ando1, Shigeyuki Magi 1, Masahide Seki2, Yutaka Suzuk
Since several chemotherapy agents act like TNF to both promote apoptosis and activate NF-B, understanding the role of NF-B in suppressing apoptosis may have significant clinical applications. To understand the effects of stimulation with TNF and the role of NF-B in regulating this response, a ...
Here, we perform single-cell CRISPRi screens of 3513 regulatory elements associated with breast cancer to measure the impact of these regions on transcriptional phenotypes. Analysis of > 500,000 single-cell transcriptomes in two breast cancer cell lines shows that perturbation of BC-associated e...
5. Five significant enrichment pathways were found simultaneously in C. albicans, C. glabrata, C. parapsilosis and C. tropicalis, which included the NF-kappa B signaling pathway, TNF signaling pathway, viral protein interaction with cytokine and cytokine receptor, salmonella infection and osteoclast ...
CALCOCO2/NDP52 is a selective autophagy receptor for xenophagy [142], and it also plays an immunosuppressive role in NFKB/NF-κB signalling through toll-like receptors (TLRs), including TLR3. The Val248Ala mutant, however, is unable to negatively regulate NFKB signalling. As CALCOCO2/NDP52...
Previous studies revealed that IL6, IL8, XIAP, and cIAP were modulated by NF-κB RelA/p65 as target genes (46., 47., 48., 49., 50.). We demonstrated that TIP60 is involved in NF-κB pathway through activating the transcriptional activity of RelA/p65 in response to TNF-α stimulation...
Interestingly, GSEA revealed that PD-L1 and PD-L2 expression correlated genes were enriched to the hallmark gene sets TNFα signaling via Nuclear factor kappa B (NFKB) (genes regulated by NF-kB in response to TNF), Kirsten rat sarcoma viral oncogene homolog (KRAS) signaling (genes up-...
Reagents. Recombinant human and mouse tumor necrosis factor (TNF)-α were purchased from Invivogen (San Diego, CA) and Peprotech (Rocky Hill, NJ), respectively. NF-κ B inhibitors, BAY11-7082 and MG-132, were purchased from Invivogen. Recombinant human interferon-α (IFN-α...
Hsa-miR-939 is predicted to target several proinflammatory genes, including IL-6, VEGFA, TNFα, NFκB2, and nitric oxide synthase 2 (NOS2A). Binding of miR-939 to the 3′ untranslated region of these genes was confirmed by reporter assay. Overexpression of miR-939 in vitro resulted in...
In this study, we set out to understand the mechanism by which NF-κB regulates cardiac dysfunction inmdxmice. We discover that unlike the typical function of NF-κB as an inducer of gene expression, NF-κB ablation inmdxcardiomyocytes causes global permissive chromatin remodeling on enhancers of...