产品报价 ￥1/48Test ￥1/96Tests 产品说明书 点击查看 购买方式 银行转账、电汇、支票、现金,在线支付宝及网银支付,或直接与我们电话联系400-6800-868 产品新闻 背景资料 USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞...
The phosphoELISA™ kit protocol (Figure 1) begins with a monoclonal antibody specific for a protein of interest (regardless of phosphorylation state) coated onto the wells of microtiter strips. Samples, including a standard containing protein of interest, control specimens, and unknowns, are pipett...
c Western blot analysis of STAT3 phosphorylation showed that AZD1480 treatment significantly reduced p-Tyr705 (sevoflurane vs propofol p = 0.03, sevoflurane vs AZD1480 p = 0.001, two-way ANOVA + Dunnett’s post hoc tests) and p-Ser727 (sevoflurane vs AZD1480 p = 0.001, ...
Western blot To examine the Nef-eGFP and eGFP expression, cells were lysed in RIPA buffer while mixing on a rotator for 30 min before sonication on ice. The samples were then centrifuged at 20,000g for 10 min and the supernatant was collected. Sample buffer (64 g l−1 SDS,...
A total of 20 μl of beads were washed twice with 1× western blot (WB) buffer (5 mM Tris-HCl, pH 7.5, 0.5 mM EDTA, 1 M NaCl, 0.02% Tween-20), once with 2× WB buffer, and finally resuspended in 40 μl of 2× WB buffer. An equal volume of sample was added...
Samples were analyzed by western blot as described above. Antibodies used for blotting were mouse monoclonal antibody to Flag (F3165; Sigma-Aldrich; 1:1,000) and rabbit monoclonal antibody to VDAC1 (#4661; Cell Signaling, Danvers, MA 1:2,000). For immunocytochemical studies, we plated COS7...
产品报价 ￥1/48Test ￥1/96Tests 产品说明书 点击查看 购买方式 银行转账、电汇、支票、现金,在线支付宝及网银支付,或直接与我们电话联系400-6800-868 产品新闻 背景资料 USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞...
you have to optimize that as this will depend on your primary Abs affinity for the Ag. In an experiment targeting CD9 we are getting strong signal on western blot after only 10 min incubation with target (both for manual & automated workflows). See more onImmunoprecipitation protocolsusing Dyn...
(G) Western blot analysis of PCBP2 protein in 786-O and A-498 cells after knockdown using two different siRNAs, as well as using a control (non-targeting) siRNA. (H) Change in the proportion of cells in different cell-cycle phases after PCBP2 knockdown in 786-O and A-498 cells. (...
(glyco-) proteins associated with malig- nant lymphoma (ML) were collected from a carcinogen-trans- formed Bloom syndrome (BS) B-lymphoblastoid cell line by means of the panning protocol, in which antigen cell clones which specifically react with antibodies in ML patient sera were separated on...