(b) The same cells were analyzed for the steady-state levels of OXPHOS proteins by western blot. To exacerbate the defect, cells were treated with different concentrations of doxycycline for 72 hours, a mitochondrial protein synthesis inhibitor. (c) Enzymatic activity of complex IV (CIV) and ...
(G) Western blot analysis of PCBP2 protein in 786-O and A-498 cells after knockdown using two different siRNAs, as well as using a control (non-targeting) siRNA. (H) Change in the proportion of cells in different cell-cycle phases after PCBP2 knockdown in 786-O and A-498 cells. (...
(glyco-) proteins associated with malig- nant lymphoma (ML) were collected from a carcinogen-trans- formed Bloom syndrome (BS) B-lymphoblastoid cell line by means of the panning protocol, in which antigen cell clones which specifically react with antibodies in ML patient sera were separated on...
the calcium activates NFAT transcription factor, and triggers gene expression of the GLP-1. GLP-1 will be released from the cells to improve glucose homeostasis. In one implementation, the illumination protocol includes irradiance of 1 mW/cm2, 5 sec on-5 sec off cycles, exposure duration of...
an improvement of ˜30 dB. We never detected ABRs for sound presented to the non-injected side. Although we did not directly analyze gene transfer in the non-injected ear, the ABR data suggest that any gene transfer to the contralateral ear is minimal using this injection protocol. Neverthel...
蛋白免疫印迹法是检测和分析蛋白的常规方法。但是,如果您想获得可重复又可靠的印迹结果,应采取一些预防措施和技巧。 在义翘神州,我们拥有超过十年的蛋白免疫印迹经验,并开发了一套技巧和诀窍,可确保生成可靠的数据和更清晰的印迹。 快速进入: • 确定目标蛋白和一抗的最佳用法 ...
低自发荧光型 Immun-Blot LF PVDF 膜 专为荧光应用优化的Immun-Blot LF PVDF 膜,使图像品质更好,并提高了所有荧光检测的灵敏度。是进行多重荧光western 转印和化学发光应用的理想选择。 主要用于红绿蓝三色荧光印迹检测,同时也适用于比色法和化学发光法。
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between the conformationally constrained protein and a protein of interest to which it specifically binds, followed by complex detection (for example, by an immunoprecipitation, Western blot, or affinity column technique that utilizes the conformationally constrained protein as the complex-forming reagent)...
Other diagnostic methods known in the clinical art include reverse transcription PCR, real-time PCR and western blot analysis. The EGFRvIII does not naturally occur in healthy human tissues. When there is a positive detection of EGFRvIII in a tumor biopsy tissue over the background detection of ...