Electronic sequence for gene synthesis Vector details (map) & cloning strategy Mail in an aliquot of your vector What you receive 4μg (1 μg for low-copy plasmid) lyophilized plasmid DNA of your insert in your chosen vector Sequence chromatograms or NGS read depth plot, plasmid construct ma...
We have several options for cloning vectors and vectors ready for protein expression experiments, in both bacteria and mammalian cells. When selecting our standard cloning vector option, if your insert is less than 250 bases we will use the pIDTSmart vector. All other lengths will be cloned ...
GenScript's GenEZ™ ORF clones are seamlessly cloned into our standard mammalian expression cloning vector, pcDNA3.1+/C-(K)-DYK, using our GenBuilder™ seamless cloning technology. Driven by a CMV promoter, pcDNA3.1+/C-(K)-DYK is equipped with a C-terminal DYKDDDDK tag for easy protein...
PURPOSE: To provide a vector for gene cloning capable of easily selecting a recombinant integrated with an exogenic DNA fragment having a recognition nicking sites of various restriction enzyme for inserting various exogenic gene. ;CONSTITUTION: The objective vector has a base sequence coding an ...
technologies, Biomatik's gene synthesis service offers cost-effective solutions and quick turn-around time for your custom DNA synthesis, cloning, and/or recombinant expression project needs. We also offer a wide range of downstream services, including subcloning, mutagenesis, and plasmid preparations....
recombinant viral vectors for the delivery of therapeutic genes into diseased tissues. While substantial progress has been made, and some clinical successes are over the horizon, further vector refinement and/or development is required before gene therapy will become standard care for any individual ...
Gene synthesis enables creation and modification of genetic sequences at an unprecedented pace, offering enormous potential for new biological functionality but also increasing the need for biosurveillance. In this paper, we introduce a bioinformatics te
Generally, it involves the insertion of a foreign DNA fragment, such as a gene, into a genetic vector to form recombinant DNA that can replicate within a host cell, such as the bacterium E. coli. From: Comprehensive Biotechnology (Third Edition), 2014 ...
DNA synthesis is an increasingly attractive approach to avoid the cloning procedure entirely. Modern technologies permit the synthesis of DNAs greater than 10,000 base pairs long, so if the desired sequence is known, a commercial company can synthesize the DNA fragment in a plasmid backbone. An ...
GenEZ™ supports direct cloning only into selected vectors. If you need cloning into a custom vector or a modified version of a commercial vector, please useSC1017 Gene Synthesis and Subcloning Bundle Service If you need codon optimization or other sequence modifications, please use our otherGen...