What is a Gene Cloning Vector? A vector is any DNA molecule which is capable of multiplying inside the host to which our gene of interest is integrated for cloning. The selection of vector depends upon the size of the fragments to be cloned. Common vectors include plasmids (Eg: pBR 322)...
1 Methods in Gene Cloning 17.3 Objectives describe the steps in gene cloning by using plasmid as the vector The steps foScreening
A vector system with temperature-sensitive replication for gene disruption and mutational cloning in streptomycetes A vector system with temperature-sensitive replication for gene disruption and mutational cloning in streptomycetes. Mol. Gen. Genet. 219:341-348... ...
the gene of interest will be cloned. Expression of the gene of interest is controlled by the human CMV promoter. The vector also contains the hygromycin resistance gene with a FRT site embedded in the 5' coding region. ...
Cloning the obtained allelic fragments into TA plasmid vectors and subsequent Sanger sequencing of 21 clones designated the sequences as specific for MSP1 (eAppendix). Comment Kinship Determination More than 55 bone biopsies were used to elucidate the individual relationships of 18th-dynasty individuals...
Bats are presumed reservoirs of diverse coronaviruses (CoVs) including progenitors of Severe Acute Respiratory Syndrome (SARS)-CoV and SARS-CoV-2, the causative agent of COVID-19. However, the evolution and diversification of these coronaviruses remains
Most patients with prostate cancer inevitably progress to castration-resistant prostate cancer (CRPC), at which stage chemotherapeutics like docetaxel become the first-line treatment. However, chemotherapy resistance typically develops after an initial p
Bottlenecks in metabolic pathways due to insufficient gene expression levels remain a significant problem for industrial bioproduction using microbial cell factories. Increasing gene dosage can overcome these bottlenecks, but current approaches suffer fr
Gene drives are genetic modifications designed to propagate in a population with high efficiency. Current gene drive strategies rely on sexual reproduction and are thought to be restricted to sexual organisms. Here, we report on a gene drive system that
Synthetic gene networks in mammalian cells are currently limited to either protein-based transcription factors or RNA-based regulators. Here, we demonstrate a regulatory approach based on circular single-stranded DNA (Css DNA), which can be used as an efficient expression vector with switchable activi...