In addition, combining feasible gene synthesis techniques with quick cloning methods into recombinant expression systems without extensive enzymatic requirements would also greatly facilitate protein structure investigations. In this study, we use a PCR-based gene synthesis technique coupled to in vivo ...
[methods in molecular biology] gene synthesis volume 852 || slic: a method for sequence- and ligation-independent cloningPeccoud, JeanLi MZ, Elledge SJ. 2012. SLIC: a method for sequence- and ligation-independent cloning. In: Peccoud J, ed. ...
Bundled with gene synthesisSubcloning Bundled with gene synthesis Service NumberSC0002SC0002SC0002 AdvantagesExpression vectors – Choose from over 150 popular vectorslistedSave on cloning into your archived vectorsGet ready to use clones in any vector, commercial or customized ...
that a combination of various technologies can be the best approach to their research. Learn about real-world applications utilizing different combinations of cloning technologies offered by Thermo Fisher Scientific to propel your research. Topics range from restriction enzymes to complete gene synthesis....
synthesis for longer gene sequences. On one hand, a longer natural gene sequence would be more likely to contain codons problematic for gene transfer, making synthesis more attractive for these sequences. On the other hand, methods and pricing for synthesis vary widely based on DNA length15, ...
Methods for assembly of highly repetitive gRNA arrays Most methods to assemble gRNA arrays do so via derivatives of popular cloning methods, such as Gibson or Golden Gate Assembly, since the repetitive sequences within gRNA arrays often make pure chemical synthesis unviable40,41. Assembly approaches...
E. coliwas used for transformation of cloning reactions using the lab strain DH5α (NEB) and cells were grown in selective Luria Bertani (LB) medium (10 g/L peptone, 10 g/L NaCl, 5 g/L yeast extract, 50 µg/mL carbinicilin) at 37 °C....
Gibson DG, Smith HO, Hutchison CA, Venter JC, Merryman C: Chemical synthesis of the mouse mitochondrial genome. Nat Methods. 2010, 7 (11): 901-903. 10.1038/nmeth.1515. Article CAS Google Scholar Gibson DG: Synthesis of DNA fragments in yeast by one-step assembly of overlapping oligonucle...
Gene knockout can be a very simple, one-step approach to eliminate a gene by substituting its DNA sequence with that of a genetic marker. From:Methods in Enzymology,2013 About this page Set alert Chapters and Articles You might find these chapters and articles relevant to this topic. ...
2. Materials and Methods 2.1. Cloning of SbERD4 Gene and In Silico Analysis The SbERD4 gene was made full length through the rapid amplification of the cDNA end (RACE) technique using an EST (Sal C38; GenBank no. EC906161) clone and gene-specific primers (Table S1). The gene was ...