This disclosure is directed to the field of polynucleotide synthesis, and the embodiments taught herein are generally directed to a kit for use in the design of a desired polynucleotide from information obtained from a polypeptide or another polynucleotide, the generation of a custom set of ...
产品名称 All-in-One™ First-Strand cDNA Synthesis Kit 产品规格 RT Reaction 有效成分含量 100% 检测方法 ELISA 样本 常规生物样本 保质期 24个月 标记物 蛋白质 储存条件 4 ℃避光保存 用途范围 适用于大部分来源的RNA进行逆转录实验 是否进口 否 包装规格 0.5kg 特性 一整套试剂盒 可售...
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RNA extraction was performed with a Quick-RNA 96 well kit (Zymo). cDNA generation was performed with a high-capacity cDNA synthesis kit using random primers and RNase inhibitor (Thermo) following recommended protocols. Quantitative RT-PCR was performed with an ABI QuantStudio 7 Flex real time ...
Total RNA was isolated following Trizol method and converted to cDNA using DSS Takara Prime Script First-Strand cDNA Synthesis kit (DSS Takara Bio India Pvt. Ltd. New Delhi, India) as per manufacturer’s instructions. cDNA was used as a template for further polymerase chain reactions (PCR). ...
英文名称:Verso cDNA Synthesis Kit 保存条件:RT 产品简介 该产品仅用于科研实验,不得用于其他用途。 相关产品 AB-0264 ABgene Strips of 8 Tubes 250 strips Abgene AB-0264/BA Bgene Strips of 8 Tubes (Blue) 250 strips Abgene AB-0264/P ABgene Strips of 8 Tubes (Purple) 250 strips Abgene ...
Coupling the gene synthesis procedure to in vivo homologous recombination techniques allows efficient subcloning and site-directed mutagenesis for error correction. The recombinant proteins of PAZ and PolA were subsequently overexpressed in E. coli and used for protein crystallization. Crystals of both ...
First strand cDNAs of the miRNAs and mRNAs were synthesized using the Mir-X miRNA first strand synthesis kit and PrimeScript RT reagent kit with gDNA eraser (Takara Biotechnology, Dalian, China) respectively. RT-PCR was performed using 2 µl of each cDNA template and the FastStart SYBR Green...
treated RNA was used to generate cDNA using the cDNA synthesis kit (Life Technologies) with random hexamers and 2 μl of cDNA was used for the GmSNAP18 quantitative PCR with the specific primers (Supplementary Table 1), using the Power SYBR Green PCR Master Mix kit (Life Technologies)....
Finally, cDNA was synthesized from 25 μg of total RNA using the SuperScriptTM Double-Stranded cDNA Synthesis Kit (Invitrogen) and a biotinylated oligo (dT)18 primer, following the conditions recommended by the manufacturer. cDNA-AFLP analysis The cDNA-AFLP technique [9] was used with some ...