In addition, the mutant alleles are likely to result in a complete loss-of-function for the gene product which, if the effect is lethal or detrimental, might limit the analysis that can be done. View article Mutation processes at the protein level: is Lamarck back? Yury O. Chernoff, in ...
SourayaJaroudi,SiobanSenGupta, inMutation Research/Reviews in Mutation Research, 2007 Gene expression patterns were similar in the yolk sac.Apng,Pms1,Pcna,Rad54,XpdandXpewere highly expressed in both tissues and the rest of the genes were expressed at low levels in the yolk sac; some were not...
Moleculargenetics is the field of biology and genetics that studies the structure andfunction of genes at a molecular level. The study of chromosomes and geneexpression of an organism can give insight into heredity, genetic variation,and... G Rounds 被引量: 2发表: 1999年 A Gain-of-Function ...
Our study provides a novel method that is used to investigate the effects of gene mutations at the cellular level. Introduction Site-directed mutagenesis is one of the most important experimental techniques in molecular biology and is widely used to investigate the structure and function of DNA and...
A constant rate of spontaneous mutation in DNA-based microbes. Proc. Natl Acad. Sci. USA 88, 7160–7164 (1991). Article CAS PubMed PubMed Central Google Scholar Elez, M. et al. Seeing mutations in living cells. Curr. Biol. 20, 1432–1437 (2010). Article CAS PubMed PubMed Central...
Targeted gene repair is a technique used to correct a mutation at a specific site in an episome or chromosome. It uses synthetic oligonucleotides together with the cell's inherent DNA repair system to direct single base pair changes. Latest Research and Reviews In vivo base editing extends life...
Expression and mutation profile of m6A-related genes in patients with CESC To analyze the overall expression of m6A-related genes in patients with CESC, we analyzed the genomic mutations and mRNA expression, including single nucleotide polymorphism, CNV, and gene expression level. SNP analysis showed...
The Mutation Generation System kit (Thermo, USA, #F701) was used to generate mutations in the AAV8 capsid ORF. Supplementary Table 1 listed all AAV8 variants. Primer pairs for mutagenesis displayed in Supplementary Table 2. Animal use Six- to eight-week-old male C57BL/6 J mice, twelve...
(Buljan and Bateman2009; Forslund et al.2011; Wu et al.2012; Gabaldón and Koonin2013). Homolog identification forms the basis for many downstream analyses in genome exploration such as analysis of trait and gene correlation, gene expression, gene functional mutation, gene ontology-based functional...
Nevertheless, this type of copy number variations (CNVs) at the single-cell level represents a new source for heterogeneity of targeted TSG mutations in CRISPR/Cas9-induced mouse liver cancer. Type and frequency of targeted mutation alterations from parental clones to subclones Given the presence ...