How noncoding DNA determines gene expression in different cell types is a major unsolved problem, and critical downstream applications in human genetics depend on improved solutions. Here, we report substantially improved gene expression prediction accuracy from DNA sequences through the use of a deep...
All of the cells within a complex multicellularorganismsuch as a human being contain the sameDNA; however, the body of such an organism is clearly composed of many different types of cells. What, then, makes a liver cell different from a skin or muscle cell? The answer lies in the way ...
what are the functional roles of different genes and in what cellular processes they participate; • how genes are regulated, how genes and gene products interact, what are these interaction networks; • howgene expression levelsdiffer in various cell types and states, how gene expression is ...
in development. Therefore, a large fly genetic toolbox has been created for reverse genetic approaches to allow cell-type and developmental-stage specific control of gene expression (Venken et al., 2011). By downregulating or increasing a specific gene's expression in specific cell-types, we ...
Gene expression profiling enables you to investigate the effects of different conditions on gene expression by altering the environment to which the cell is exposed, and determining which genes are expressed. Alternatively, if you alrea...
Yes, the module score represents relative expression. If gene A is highly expressed across all cells, the module scores assess whether a given cell expresses gene A more often than other highly expressed genes. Therefore, if the module score is high, it indicates these genes are expressed. If...
After examining mouse, rat and human breast cancer cells, they found that ZBP1 silencing occurs when a methyl group attaches to ZBP1's promoter region (the segment of a gene where gene expression is initiated). The attachment of methyl group prevents the promoter from binding to a protein ca...
MARBLES Accounts for known cell type relationships as part of the differential expression analysis, after first pseudobulking the data. Since there are generally known relationships between the different cell types in a dataset (for example, related through a hierarch...
(especially in higher eukaryotes) • Eukaryotic cells contain 5000 or more different polypeptides, some of which are very rare • Particular cell types or tissues may contain several dozen abundant proteins not readily detected in other cell types 1 2 • The central dogma: ➔RNA ➔ ...
DNA is found as a single circularchromosomein thecytoplasm. Some prokaryotes, such asbacteria, and a few eukaryotes have extrachromosomal DNA known asplasmids, which areautonomous, self-replicating genetic material. Plasmids have been used extensively inrecombinant DNA technologyto study gene expression....