选择GAS6-AS1表达水平最高的LoVo细胞和GAS6-AS1表达水平最低的HT29细胞进行体外实验。为了研究GAS6-AS1是否影响CRC细胞的活力和迁移,研究者在HT29细胞中过表达GAS6-AS1(图2B),并在LoVo细胞中抑制GAS6-AS1的表达(图2C)。CCK8...
(P<0.05),细胞凋亡率显著升高;下调GAS6-AS1表达后并用6 Gy X射线照射细胞,活力明显降低(P<0.05),细胞凋亡率明显升高.GAS6-AS1 wt+miR-324-3p mimics组荧光素酶活性显著低于mimics NC+GAS6-AS1 wt组(P<0.01).与癌旁正常组织比较,脑胶质瘤组织中miR-324-3p mRNA表达显著降低(P<0.01).2,4,6 Gy X...
GAS6-AS1 increased the 50% inhibiting concentration of 5-FU, enhanced cell proliferation and accelerated G1/S transition, both with and without 5-FU, both in vitro and in vivo. Mechanistically, GAS6-AS1 enhanced the stability of MCM3 mRNA by recruiting PCBP1, consequently increasing MCM3 ...
1C). We also studied the GAS6-AS1 expression by analyzing TCGA and Genotype-Tissue Expression (GTEx) database, as tumor and normal controls, respectively. The GAS6-AS1 expression was higher than all the other cancers (Fig. 1D). Additionally, comparing AML and normal controls (TCGA vs GETx...
Conclusion: These findings suggested that low expression of GAS6-AS1 might exert an essential role in AFST through downregulating its downstream target mRNAs GOLGA8A and BACH2, and GAS6-AS1 might be a potential target for AFST therapy.
采用RT-qPCR法检测lncRNA GAS6-AS1,IL-11及纤维化标志物α平滑肌肌动蛋白(α-SMA),Ⅰ型胶原蛋白α1(COL1A1)mRNA表达.另取传3～6代,对数生长期,生长状态良好的ESCs,分别转染三条si-GAS6-AS1序列以及si-Control序列,采用RT-qPCR法验证转染效率.将转染si-GAS6-AS1的ESCs用无血清培养基饥饿培养后予TGF-β1...
目的:本研究旨在找出在结直肠癌(Colorectal Cancer,CRC)中差异表达且具有临床意义的目标lncRNA;并通过进一步的体外细胞实验明确长链非编码RNA GAS6-AS1对结直肠癌细胞的增殖,迁移及侵袭能力的影响.方法:1.收集并使用从TCGA数据库检索并下载的结直肠癌样本(包含了 647例结直肠癌组织和51例癌旁组织)的RNA-seq数据和...
lncRNA GAS6-AS1靶向调控IL-11对子宫 内膜基质细胞向成纤维细胞分化的影响doi:10.3969/j.isnn.1002-266X.2023.13.003巫剑红代荫梅田玉翠蒋子雯王静璇张宇迪Shandong Medical Journal
GAS6-AS1GLUT1glucose metabolismlung adenocarcinomaGlucose metabolism reprogramming is one of the hallmarks of cancer cells. While functional and regulatory mechanism of long noncoding RNA (lncRNA) in the contribution of glucose metabolism in lung adenocarcinoma (LUAD) remains incompletely understood. The...
GAS6-AS1表达水平.Kaplan-Meier法分析组织lncRNA GAS6-AS1表达与子宫内膜癌患者预后的关系,多因素Cox回归分析影响子宫内膜癌预后的因素.结果与癌旁组织相比,子宫内膜癌组织中lncRNA GAS6-AS1表达水平较高(P<0.001).lncRNA GAS6-AS1表达水平与浸润深度,盆腔淋巴结转移具有相关性(P<0.05).lncRNA GAS6-AS...