Rosslyn, VA: National Electrical Manufacturers Association, 2004. Google Scholar Zanzonico P, Heller S. The intraoperative gamma probe: basic principles and choices available. Semin Nucl Med 2000;30:33–4. Article PubMed CAS Google Scholar Daghighian F, Mazziotta JC, Hoffman EJ, Shenderov ...
In the earliest days of nuclear medicine, counting devices similar to the thyroid probe described in the previous section were used to evaluate the amount of activity in a particular tissue. For example, probes could be used to evaluate the iodine uptake of thethyroid gland. However, it was ...
Club Cells can be Multi-Functional Club cells by definition secrete SCGB1A1.45,46To investigate if the same cells that produced SCGB1A1 also secreted BPIFA1, we used a combination of RNA-ISH and IH to determine co-localization. An RNA probe forBpifa1was used followed by detection of SCGB1A...
Compared to conventional probe detectors, the small gamma-camera has advantages of providing both spectrometric and spatial information. A portable imaging system may also give possibilities for pharmacokinetic studies, where its ease of access can enable more frequent measurements than regular camera ...
Accumulation of PCR products was detected in real time by monitoring the probe cleavage-induced mobilization of the reporter dye. ELISA and cell-based ELISA Cells were cultivated for 3 h – 72 h as indicated in the Results section. Subsequently, cell culture supernatants were collected and cells...
PS1 and Nicastrin (Nct) Western blots were employed as readout for association of the gamma-secretase complex with the biotinylated probe. Displacement of the active site isostere by test compounds resulted in lowered intensity of PS1 and Nct bands on the immunoblots. The biotinylated active-site...
(Enzo Life Sciences, Farmingdale, NY, USA) according to the manufacturer’s protocol. The cells were seeded in a 96-well cell culture plates at 104cells per well and cultivated for 24 h, followed by 24 h experimental treatment. Then, the cells were stained with ROS/superoxide probe mixture...