In this chapter we discuss this explosion of data, the implication it has upon our understanding of the nature of GalNAc-type O-glycosylation, and the development of bioinformatic tools to aid our comprehension of the O-glycoproteome. As tools to aid comprehension, predictors of O-glycosylation ...
Control of mucin-type O-glycosylation: A classification of the polypeptide GalNAc-transferase gene family Glycosylation of proteins is an essential process in all eukaryotes and a great diversity in types of protein glycosylation exists in animals, plants and m... EP Bennett,M Ulla,C Henrik,......
COMMUNICATIONS | DOI: 10.1038/ncomms7937 Mucin-type (GalNAc-type) O-glycosylation is by far the most differentially and complex regulated type of protein glycosylation, and likely the most abundant with over 80% of all proteins passing through the secretory pathway that is predicted to be O-...
glycosylationglycosyltransferaseinducible expressionmass spectrometry (MS)The GalNAc-type O-glycoproteome is orchestrated by a large family of polypeptide GalNAc-transferase isoenzymes (GalNAc-Ts) with partially overlapping contributions to the O-glycoproteome besides distinct non-redundant functions. Increasing...
Protein glycosylation is one of the most common protein modifications. A major type of protein glycosylation is O-GalNAcylation, in which GalNAc-type glycans are attached to protein Ser or Thr residues via an O-linked glycosidic bond. O-GalNAcylation is
Engineering mammalian mucin-type O-glycosylation in plants. (NAc) C4-epimerase and a human polypeptide GalNAc-transferase in leaves of Nicotiana benthamiana resulted in GalNAc O-glycosylation of co-expressed human O... Z Yang,DP Drew,B Jorgensen,... - 《Journal of Biological Chemistry》 被引量...
Mucin-type O-glycosylation is initiated by a large family of UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (GalNAc-Ts) that target different proteins and are differentially expressed in cells and organs. Here, we investigated the expression patterns of all of the GalNAc-Ts in colon ...
occurs on serine/Threonine (Ser/Thr) of peptide chain, and the sugar chain and Ser/Thr side chain hydroxyl generate O - Glycosidic bond under enzyme catalysis. A common type of O-glycosylation modification starts with GalNAc, forming O-GlacNAc bonds, known as mucin like O-glycosylation....
The glycosylation of serine and threonine residues during mucin-type O-linked protein glycosylation is catalyzed by a family of polypeptide GalNAc transferases (EC 2.4.1.41). Five distinct GalNAc-transferase genes termed GalNAc-T1, -T2, -T3, -T4 and -T5 have been cloned and characterized. Homa...
但是,与目前相对较为成熟的N连接糖基化和O连接氮乙酰葡萄糖糖基化(O-GlcNAc)分析方法相比,O-GalNAc相关研究还比较少(文献2.Chen,R.,Seebun,D.,Ye,M.,Zou,H.,Figeys,D.,Site-specificcharacterizationofcellmembraneN-glycosylationwithintegratedhydrophilicinteractionchromatographysolidphaseextractionandLC-MS/MS....