通过整合RNA-seq,ATAC-seq及Hi-C的多组学数据分析,研究人员发现SOCS1是G-CSF动员后表达水平上调最为显著的基因,而转录因子STAT3可能是直接调控SOCS1表达的上游因子(图2A)。进一步整合分析原代T细胞中增强子信号(H3K27ac)以及STAT3的染色质...
以上结果提示,T细胞缺失Socs1后G-CSF对GVHD小鼠的保护作用消失,证明G-CSF在体内通过上调T细胞中SOCS1水平从而诱导T细胞进入免疫耐受态,从而降低GVHD损伤。进一步研究发现,SOCS1不仅对CSF3R/JAK2/STAT3信号通路有抑制作用,同时对NLRP3炎症小体信...
通过整合RNA-seq,ATAC-seq及Hi-C的多组学数据分析,研究人员发现SOCS1是G-CSF动员后表达水平上调最为显著的基因,而转录因子STAT3可能是直接调控SOCS1表达的上游因子(图2A)。进一步整合分析原代T细胞中增强子信号(H3K27ac)以及STAT3的染色质结合(CUT&Tag)数据发现,G-CSF可能通过重构SOCS1基因编码区的染色质三维结构上...
揭示G-CSF的生物学功能与机制 G-CSF通过结合受体(G-CSFR)激活JAK-STAT、MAPK等信号通路,调控细胞增殖、凋亡及免疫应答。利用ELISA试剂盒,研究人员可结合基因敲除、药物干预等手段,分析G-CSF与下游分子(如STAT3、SOCS3)的关联性,深入探究其在免疫调控或肿瘤微环境中的作用机制。 支持药物研发与疗效评估 升白药物评价...
综上,研究人员通过多组学分析并结合体内及体外功能实验,完善了G-CSF诱导T细胞免疫耐受的分子机制模型:G-CSF通过与T细胞表面受体CSF3R结合,激活STAT3。活化的STAT3进入细胞核,通过重塑SOCS1基因编码区染色质三维结构,上调SOCS1的表达水平。高水平SOCS1不仅可以负反馈抑制CSF3R/STAT3通路,同时还可抑制NLRP3炎症小体信号通...
In hematopoietic cells from Stat3-deficient mice, trace levels of SOCS3, a negative regulator of granulopoiesis, were observed, and SOCS3 expression was not induced by G-CSF stimulation. Stat3-null bone marrow cells displayed a significant activation of extra-cellular regulated kinase 1 (ERK1)/...
(Jak2-STAT3) pathway, thereby promoting survival, proliferation, differentiation and mobilisation of haematopoietic stem and progenitor cells. G-CSF is a cytokine that have been demonstrated to improve cardiac function and perfusion in myocardial infarction. And it was initially evaluated as a stem ...
(Jak2-STAT3) pathway, thereby promoting survival, proliferation, differentiation and mobilisation of haematopoietic stem and progenitor cells. G-CSF is a cytokine that have been demonstrated to improve cardiac function and perfusion in myocardial infarction. And it was initially evaluated as a stem ...
transgenic mice with a targeted mutation of the G-CSF receptor that abolishes G-CSF–dependent Stat3 activation show severe neutropenia, with an accumulation of immature myeloid precursors in the bone marrow ; constitutively active Stat3 partially rescues this neutropenia. Stat3 is then thought to tran...
Consistent with this notion, we were unable to detect Csf3r mRNA in DRG neurons, in agreement with studies using single-cell (41) and genome-wide RNA sequencing (42), and could not demonstrate enhanced intracellular Ca2+ levels, as well as ERK-1/2 and STAT3 phosphorylation, in these ...