Electrophoresis mobility-shift and DNase I footprinting assays demonstrated that PGl protein binds preferentially to the G-box (CACGTG) and not to other two phaseolin E-box motifs (CACCTG and CATATG). Expression of PGl RNA was detzble in all stages of seed development as well as in flower...
GUS reporter expression from 11 basal promoters (CaMV –90) with G-box cores (CACGTG) was analysed to evaluate the regulatory roles of G-box flanking sequences. While most G-box motifs exhibited some tissue preference of gene expression, a distinct tissue-specific expression was not apparent....
Another common feature of MeJA-responsive promoters is the presence of a G-box (CAC- GTGG) or G-box-like motifs. For example, G- boxes have been identified in JA-responsive pin2 gene promoter of potato (Kim et al., 1992), the vspB gene promoter of soybean (Mason et al., 1993),...
ABSTRACT Plants have significantly more transcription factor (TF) families than animals and fungi, and plant TF families tend to contain more genes—these expansions are linked to adaptation to environmental stressors (1, 2). Many TF family members bind to similar or identical sequence motifs, suc...
DNA-binding protein CAN binds three CANNTG motifs, CACGTG (−248/−243), CACCTG (−163/−158), and CATATG (−100/−95). Substitution mutation of the CACGTG motif, which is commonly known as G-box, reduced the −295 promoter activity by 75%, indicating that the G-box ...
In tomato, SlHY5 can bind to promoters of SlCHS1, SlCHS2, and SlDFR at sites containing G-box and ACE motifs to promote anthocyanin biosynthesis (Liu et al., 2018). Show abstract Genome-wide characterization of RsHSP70 gene family reveals positive role of RsHSP70-20 gene in heat stress...
We also identified many conserved protein motifs that suggest a modular structure for GBFs. At the whole plant level, GBFs have been shown to be involved in developmental and physiological processes in response to major cues such as light or hormones. Nevertheless, it remains difficult to assign...
(Fig.7). Among the examined DNA motifs, a core region containing CG is the dominant motif, suggesting the G-box (5′-CACGTG-3′) binding preference of AtPIF3–bHLH, which prefers CG, AA, CA, and AG, in descending order. However, we also observed an exception: the tandem E1-E2 ...
Another feature of G-patch proteins that is of great significance for their mode of activation is that the G-patch motifs are embedded in intrinsically disordered regions and are themselves devoid of a stable fold (Christian et al. 2014). In the absence of a binding partner, they have only...
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