An interesting extension of this approach is the inclusion of a cleavable linker between the protein domains of interest. The 2A proteinase of the foot and mouth disease virus (FMDV), a short peptide that has th
et al. Targeted therapies in HER2-positive breast cancer with receptor-redirected Arazyme-linker-Herceptin as a novel fusion protein. Breast Cancer 31, 1101–1113 (2024). https://doi.org/10.1007/s12282-024-01625-y Download citation Received15 December 2023 Accepted04 August 2024 Published09 ...
A cryo-electron microscopy structure of the Cap2–CD-NTase complex reveals Cap2 as an all-in-one ubiquitin transferase-like protein, with distinct domains resembling eukaryotic E1 and E2 proteins. The structure captures a reactive-intermediate state with the CD-NTase C terminus positioned in the ...
Phycobiliprotein as fluorescent probe and photosensitizer: A systematic review 2021, International Journal of Biological Macromolecules Citation Excerpt : The methods have less affected by other factors. Wu prepared allophycocyanin as immune probe by BAS method, which reduced the lowest limit to 0.11 ng...
The affinity of CBD proteins for cellulose can depend on several factors: the structure of the CBD, the linker between the CBD and the target protein, the pH of the solution, ionic strength, temperature, and the ratio of protein-CBD concentration to cellulose [43]. Currently, there are ...
Escherichia coli is the most widely used heterologous protein expression system. However, this system remains a challenge due to the low solubility of prot
At 5 days post-transfection, cell culture supernatant was harvested and purified with a Protein A column (GE Healthcare, Chicago, IL). The antibody was eluted using IgG Elution Buffer (ThermoFisher Scientific, Waltham, MA) and brought to neutral pH with 1 M Tris-HCl, pH 8.0. Eluted ...
33 Gel filtration and ultracentrifugation studies of additional ATIC deletion mutants suggest that the linker region contains a dimerization domain (G. P. Beardsley, personal communication). The first 229 amino acid residues of the predicted ATIC-ALK protein are identical to those of ATIC (the ...
These linker peptides may be inserted between one or more polypeptides which make up each of the fusion proteins. DEFINITIONS “Fusion polypeptide” or “fusion protein” refers to a protein having at least two heterologous Mycobacterium sp. polypeptides covalently linked, either directly or via an...
The present invention is directed to novel targeted heterodimeric fusion proteins comprising an IL-15/IL-15Rα Fc-fusion protein and a TIM-3 antibody fragment-Fc fusion protein.