The fusion protein linker 的选择 蛋白融合Linker的设计与选择 接头序列(Linker)设计是基因融合技术能否成功的关键技术之一。即通过一段适当的核苷酸序列将不同的目的基因连接起来, 使其在适当的生物体内表达成为一条单一的肽链, 其中起连接作用的氨基酸称为Linker[1]。融合蛋白中的两种成分能否分别形成正确的空间结构、...
This review covers the current knowledge of fusion protein linkers and summarizes examples for their design and application. The general properties of linkers derived from naturally-occurring multi-domain proteins can be considered as the foundation in linker design. Empirical linkers designe...
protein technology. This reviewwill summarize the current knowledge of linker design in recombinant fusion proteins. First, an overview of the properties of linkers in naturally-occurring multi-domain proteins will be provided as a general reference for linker design. Next, the empirical linkers ...
There are essentially two types of fusion proteins: the first of which consists of two proteins or protein subunits fused end-to-end and usually linked by a linker, and the second, in which amino acids from both donors are interspersed in the fusion protein product. ...
fusion protein is comparable to those of the individual components. A potential risk of this approach is that the fusion site might give rise to an immunogenic peptide. An interesting extension of this approach is the inclusion of a cleavable linker between theprotein domainsof interest. The 2A ...
Taking into account the flexibility of the relative positions of SBD and ND, the length of the linker would be enough long to cause the conformational change of TDnaK and represent the structures of the models in Fig. 6. This reconfirmed the adequacy of the linker used here. The TK-B ...
fusion protein to GPCRs with two extended helices, typically TM5 and TM6. A single extended helix at one linker site connecting the GPCR to the fusion protein does not likely yield sufficient rigidity to drive image alignment for high-resolution structure determination, particularly when the other...
Design of inter-peptide linkerBivalent, bispecific single-chain antibody fusion protein construction appears to be a promising tool for tumor therapy. One of its drawbacks is that the function and activity of the fusion proteins have varied affinity and/or anti-tumor activity compared with the ...
It can be accessed through the Web page http://www.fccc. edu/research/labs/feng/linker.html. 展开 关键词: fusion protein linker sequence loop library program proteins DOI: 10.1093/protein/13.5.309 被引量: 189 年份: 2000 收藏 引用 批量引用 报错 分享 ...