One important element in our understanding has been the ability to begin to see where proteins are in cells and what they are doing as they go about their business. Accordingly, there is now a strong impetus to discover new ways of looking at the workings of proteins in living cells. ...
Cells also secrete various proteins that become part of the extracellular matrix or are involved in intercellular communication. Proteins are sometimes altered after translation and folding are complete. In such cases, so-called transferase enzymes add small modifier groups, such as phosphates or ...
— A chemical probe should be a potent and selective ligand of its target protein that modulates the biochemical activity of the target in cells. Ideally, it should have well-characterized selectivity, especially with respect to closely related proteins. It should be active in cells at a reasonab...
We found that all 17 nGAPs were highly concentrated in the growth cones of PC12D cells, and that knockdown of all of them by RNAi reduced or inhibited neurite outgrowth, indicating that all of the 17 nGAPs may be general growth cone markers. Among them, eight proteins were shown to ...
8. Membrane proteins: proteins in the various membrane structures of cells. 9 、 carrier protein: a transmembrane protein involved in the transport of substances in the membrane structure. The carrier proteins in the cell membrane are specific in assisting diffusion and active transport. 10. ...
Recent chemical proteomic studies suggest that more than 100 proteins are myristoylated in human cells (Thinon et al., 2014). Typically, myristoylation enhances protein-membrane association that is required for target protein proper localization and biological function. S-Palmitoylation (or S-acylation)...
Secondary Structures of Proteins. Secondary structures arise from non-covalent interactions between amino acids across the chain. There are only Figure 1. Structure of an amino acid. two bonds that can rotate in space between each amino acid in the backbone of the primary sequence. These res...
Thrombospondin (Thbs) proteins are produced by the body in cells where tissues are being injured, reconfigured or remodeled, such as in chroniccardiac disease. They appear in part of the cell's internal machinery called theendoplasmic reticulum. There, Thbs triggers a stress response process to ...
DNA- and RNA-binding proteins (DRBPs) constitute a significant fraction of cellular proteins and have important roles in cells. Their functions include control of transcription and translation, DNA repair, splicing, apoptosis and mediating stress responses. ...
www.nature.com/scientificreports OPEN Suppressing gain‑of‑function proteins via CRISPR/Cas9 system in SCA1 cells Mariangela Pappadà 1, Ottavia Bonuccelli 1, Mattia Buratto 1, Riccardo Fontana 1,2, Mariaconcetta Sicurella 3, Anna Caproni 1, Silvia Fuselli...