Current single-cell RNA sequencing (scRNA-seq) methods with high cellular throughputs sacrifice full-transcript coverage and often sensitivity. Here we describe Smart-seq3xpress, which miniaturizes and streamli
Despite the increasing number of epigenomic studies in plants, little is known about the forces that shape the methylome in long-lived woody perennials. The Lombardy poplar offers an ideal opportunity to investigate the impact of the individual environme
The roar of the tiger is as menacing as if you'd heard one in real life, as is the grumbling of the alligator. The hissing of the Ball python as it moves around gives off the feeling that it's about the strike at any moment. There are some cute ones, too, like the adorable bar...
Frozen pellets were then thawed at 4 °C and lysed by passing them through a cell homogenizer containing a 4 μm-clearance ball bearing (Isobiotec, Germany) 20 times by hand or using a custom build machine [31]. The whole-cell lysate was then centrifuged at 4 °C at 16,000g ...
lactis strains, grown under conditions simulating the temperature and oxygen availability of the human gut. The experiments revealed that the L. lactis ssp. lactis Lc4 strain exhibited the strongest impact on the HT-29 cell line (Supplementary Fig. 1a). We further examined the effect of L. ...
(pubertal; pedicle growth has initiated; and FP was sampled at this stage, as we believe FP should still be comparatively quieter to the activated AP); the DoPP and AcPP were from three 2-year-old deer (first round of antler regeneration from the fully-grown pedicles). Methods for the ...
Super-resolution images were converted to 8-bit grayscale, and the background noise signals were subtracted with the rolling ball background subtraction (radius, 50 pixels) of ImageJ (v1.53j, https://imagej.net/Fiji). The methods for clustering analysis of nucleosomes were same as previous ...
Scutellaria, a sub-cosmopolitan genus, stands as one of the Lamiaceae family’s largest genera, encompassing approximately 500 species found in both temperate and tropical montane regions. Recognized for its significant medicinal properties, this genus h
Guanine-rich sequences are able to form complex RNA structures termed RNA G-quadruplexes in vitro. Because of their high stability, RNA G-quadruplexes are proposed to exist in vivo and are suggested to be associated with important biological relevance. H
(Ball and (Ball & Shivakumar,2005). Information on profits and their components is important for stakeholders in measuring business performance and forecasting future cash flows (Shubita,2021). Factors that might influence the quality of financial statement reporting include the quality of government...