Current single-cell RNA sequencing (scRNA-seq) methods with high cellular throughputs sacrifice full-transcript coverage and often sensitivity. Here we describe Smart-seq3xpress, which miniaturizes and streamli
Full size image Assessment of the impact on laccase production of affected proteins We next sought to select a few targets shown to be depleted in the proteomic analysis to assess their potential impact on heterologous protein production. We selected several targets based on GO term enrichment, nam...
Full size image We then analyzed the microbiome structure along the gastrointestinal tract using PCoA and PERMANOVA based on weighted unifrac and Bray-Curtis distance metrics. Distinct separation was observed in community structure between the small intestine and large intestine regardless of dietary treat...
The sorghum linesAdoMetase#1 and #3 were grown in the field until the soft dough stage in two different locations to evaluate their performance under natural environment. A composition analysis was performed on extractive-free cell wall residues obtained from stems and leaves. InAdoMetase#1, the ...
Full size image Table 1 Leaf samples collected for the first methylome study from individual Lombardy poplar ramets Full size table In the second methylome study (Fig. 2B), we investigated whether methylome variation within plants grown in a non-parental environment correlates with the parental clima...
Full size image Typically, theScutellariachloroplast genomes contained 112–115 coding genes, including 79–81 protein-coding genes, 29–30 transfer RNA (tRNA), and four ribosomal RNA (rRNA) genes, arranged in the same sequence (Additional file 1: Table S1). Most protein-coding genes were pres...
used. Each line was scanned 3 times and the signal was accumulated. The typical image size for purified SVs was 50 mm2. Labeling of neurons using DNA PAINT Hippocampal culture neurons (21 DIV), grown on 24-mm cover slips, were fixed using 4% PFA for 15 min. Then, the coverslips...
Accelerated evolution of TBX5 was shown in green ball in phylogenetic tree. Numbers in parentheses represented the number of species used in this study Full size image Table 1 Two-ratio model estimating ω values in 16 limb-related genes in mammals Full size table Analysis of cis-regulatory ...
Guanine-rich sequences are able to form complex RNA structures termed RNA G-quadruplexes in vitro. Because of their high stability, RNA G-quadruplexes are proposed to exist in vivo and are suggested to be associated with important biological relevance. H
(pubertal; pedicle growth has initiated; and FP was sampled at this stage, as we believe FP should still be comparatively quieter to the activated AP); the DoPP and AcPP were from three 2-year-old deer (first round of antler regeneration from the fully-grown pedicles). Methods for the ...