Tests for Anti-HCV, though they may come in the rapid form of LFAs, cannot determine active infection. Thus, after Anti-HCV testing, which can take from 20 min to 3 days depending on the type of test used to return a result, an additional sample must then be taken for NAAT testing....
In an effort to overcome the harsh GI microenvironment, these composites were prepared using biodegradable polymers including guar gum, chitosan, and poly(vinyl alcohol), in combination with montmorillonite clay as a nano-filler, using a cross-linker in the form of tetraethyl orthosilicate. In mice...
Detection of interleukin 17 (IL-17) receptors (R) IL-17R A and IL-17R B on mouse Schwann cells (SCs), using immunocytochemistry and rtPCR analysis. (A-C) immunocytochemistry for IL-17R A, IL-17R B with mainly membrane-bound expression (D-F). (G-I) Real-time PCR results of IL-17...
B. Alternate strategy for CTF4 knockdown using a heat-shock promoter upstream of the RNAi sequence. Analysis by rtPCR shows an 81% reduction in CTF4 transcript compared to wild-type controls. Full size image Ctf4 is required for viability Using the Actin promoter driven Gal4 driving ...
of human BMP-7 cDNA were obtained by reverse transcription (RT)-PCR.Following application,the two segments were ligated to each other and subcloned into PGEM-T easy vector to form PEGM-T easy/hBMP-7 recombinant plasmid.Sanger dideory chain-ter-mination method was used to sequence the ...
B: Graphic representation of the localization of the multicopy Spin gene family on the mouse X chromosome. C: Comparision between the array data and Q-RTPCR data of Ssty1 mRNA expression in two batches of wild type and Hr6b knockout spermatocytes and spermatids. Full size image Overall ...
Expression of PDGFRα and PDGFRβ isoforms was verified in pBSMC by real-time RT-PCR and immunoblot analysis (Figure S2A & S2B (see Additional file 1)). Cells subjected to triplex SILAC labeling were treated with 1 nM PDGF-BB for 0, 4 or 24 h. Total protein lysates were analyzed ...
Microarrays and related technologies such as RT-PCR have accelerated our ability to understand the effects of long chain polyunsaturated fatty acids (LC-PUFA) and their derivatives on the transcriptome, implied metabolome, and lipid signaling cascades in various species and tissues [1, 2]. Transcript...
Transcript abundance profiles in both experiments for nine selected known and putative wheat transcription factors were independently confirmed by real time RT-PCR. These expression profiles confirm or extend our knowledge of the roles of the known transcription factors and suggest roles for the unknown...
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