Reverse transcription-polymerase chain reaction; RT-PCR A method of polymerase-chain-reaction amplification of nucleic acid sequences that uses RNA as the template for transcribing the corresponding DNA using reverse transcriptase. Mentioned in:Noroviruses ...
RT-PCR Sets New Trends in Medical ResearchReverse Transcriptase-Polymerase Chain ReactionMedical ResearchGene ExpressionMolecular Diagnostic ToolsSnehalatha SalianExpress Pharma Pulse
研究者采用基于TaqMan探针的实时定量RT—PCR(RQ—PCR)技术检测195例白血病患者骨髓细胞融合基因转录子水平,其中M—bcr—abl^+慢性粒细胞白血病慢性期(CML—CP)50例、M—bcr—abl^+急性淋巴细胞白血病(ALL)10例、m—bcr—abl^+ALL19例、TEL—AML1^+ALL11例、AML1-ETO^+急性髓系白血病(AML)30例、PML—RARα...
肿瘤微转移的定量检测不仅有助于肿瘤微转移的早期诊断、判断预后,而且为临床指导化疗提供有效的依据.本文主要论述荧光定量RT-PCR技术检测肿瘤微转移的原理、基本程序、提高定量检测准确性的一些手段及其临床意义. 著录项 来源 《医学综述》 |2007年第3期|231-233|共3页 作者 乔建梁; 孟兴凯; 张俊晶; 孙娟; ...
细分层面来看,报告按产品种类、终端应用及地区进行细分分析,研究范围包括各细分领域市场占比、市场规模及增长趋势。按种类划分,RT-PCR试剂盒行业可细分为igM公司, 其他。按最终用途划分,RT-PCR试剂盒可应用于科学研究, 诊断中心, 医院等领域。 中国RT-PCR试剂盒行业主要企业有Sansure, Shanghai BioGerm Medical Biotec...
@@ 甲胎蛋白(AFP) mRNA是肝组织的一个特异性表达基因,近年来发现其在部分肝癌患者外周血中呈阳性表达,引起了许多学者的关注[1,2].我们采用实时定量逆转录聚合酶链反应(RT-PCR)技术对不同肝病患者的外周血AFP mRNA进行了定量检测,以探讨其在诊断肝癌微转移病灶的可行性. ...
Daily Percentage of Positive SARS-CoV-2 Tests in Participants With Reverse Transcription–Polymerase Chain Reaction (RT-PCR)–Confirmed Infection View LargeDownload Daily percentage of positive SARS-CoV-2 tests (lines) and 95% CIs (shaded areas) of RT-PCR tests, home antigen tests, and viral ...
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HBV reactivation post-liver transplantation can result in a post-transplant mortality rate of up to 50% within two years, making prophylaxis critical. Currently, a combination therapy of HBIG and nucleotide analogues is commonly used. Among the nucleotide analogues (NA), entecavir (ETV) and tenofo...
Full size image Multiplex assay development, analytical sensitivity and clinical validation A multiplex assay format was developed that would allow 93 samples to run in a single 96-well PCR plate by detection of all 3 targets in a single well while minimizing changes to the existing singleplex as...