Following a 96-hour incubation period at 37 °C, the migration of cells towards the wound site was captured using light microscopy, and the migration area was quantified using Image J software. (2) For tumor invasion assay, MCF-7 cells were cultured to form spheres in hanging drops of ...
Kinase signaling pathways represent the most common form of reversible post-translational modifications that control many aspects of cellular function. Aberrant activation of protein kinases drive major hallmarks of malignancies, including alterations in cellular proliferation, survival, motility and metabolism,...
Additionally, immunofluorescence, western blotting, and reverse transcription polymerase chain reaction were used to evaluate changes in the expression of Bcl-2 and Bax in the mouse hippocampus, and SIRT1/PGC-1α signaling pathway proteins were detected. Moreover, the oxidative stress markers in ICR...
cells coexpressing Bcl-XL-CFP and Beclin-1-YFP, we showed that Bcl-XL bond to Beclin-1 to form heterooligomers whose stoichiometry increases from 1:1 to 2:1 or higher with the increasing relative expression level of Bcl-XL, indicating the multiple binding sites of Beclin-1 with Bcl-XL....
The biological effects observed in the aforementioned PDNVs form the basis for their tissue protection and repair properties. However, some studies have only conducted a superficial assessment of the PDNVs' activity without delving into the specific underlying mechanisms. It remains unknown whether the...
a unique form of programmed cell death (PCD) distinct from apoptosis, necrosis, and others, has emerged as a key area of interest. However, comprehensive reviews on how natural products influence ferroptosis in respiratory diseases are lacking. This review will explore the therapeutic potential and...
The paralog of BCL11A, BCL11B, is required for T cell development [77]. As a result, it can have either tumor suppressive or oncogenic functions in different hematological malignancies. In T-ALL,BCL11Bmay be a haploinsufficient tumor suppressor gene, in part because it may help maintain a ...
form of dual therapies, is variable. Toxicity resulting from their non-selectivity due to AKT, ubiquitous expression [11] and structural homology with other functionally important proteins [2,3,4,12], and the development of resistance because of crosstalk between AKT and a plethora of other ...
Medicinal plants have been used for centuries as remedies for human diseases [1], and ancient medication was recently accepted as an alternate form of health care. As various infective agents are r...
Western blot analysis of sucrose gradient-purified influenza viruses and a recombinant RBD protein using:Aanti-RBD rabbit polyclonal antibody (*) – influenza HA monomer with RBD insertion; (♦) – monomeric recombinant RBD (♦♦) – dimeric form of RBD; the higher bands are oligomers of ...