Antibody-dependent cellular cytotoxicity (ADCC) is promoted through interaction between the Fc region of immunoglobulin G1 (IgG1) and Fcγ receptor IIIa (FcγRIIIa), depending on-glycosylation of these glycoproteins. In particular, core fucosylation of IgG1-Fc-glycans negatively affects this ...
against tumor growth due to enhanced antibody-dependent cellular cytotoxicity (ADCC) with LS174T colorectal carcinoma and A20 lymphoma cells12. These inhibitors enter via the fucose salvage pathway yet it is estimated that ~90% of the GDP-Fuc pool is biosynthesized via de novo biosynthesis from ...
Afucosylation and elevated galactosylation in the Fc region of the HB3VAR06-specific IgG1 increased NK-cell mediated ADCC (Fig.6a, c, d), as did afucoslyation of HB3VAR06-specific IgG3/4 (Fig.6e), likely due to their improved binding affinity for FcγRIIIa28. Moreover, C1q-binding...
As an example, recombinant antibodies derived from Chinese hamster ovary (CHO) cells are generally highly fucosylated; the absence of fucose significantly enhances antibody dependent cell-mediated cytotoxicity (ADCC) against cancer cells. This chapter describes a protocol applying clustered regularly ...
However, the most dramatic effect on ADCC activity is observed when two carbohydrate chains are present rather than one, regardless of afucosylation state... W Shatz,S Chung,B Li,... - 《Mabs》 被引量: 60发表: 2013年 Knobs-into-holes antibody production in mammalian cell lines reveals tha...
The extent of afucosylation, which refers to the absence of core fucose on Fc glycans, can correlate positively with the antibody‐dependent cellular cytotoxicity (ADCC) activity of a monoclonal antibody (mAb). Therefore, it is important to maintain consistent afucosylation during cell culture proces...
Such hypofucosylated or nonfucosylated antibodies may find use, for example, in treatment of human disease in which is it therapeutically beneficial to direct antibody dependent cellular cytotoxicity (ADCC) mediated killing of cells expressing the antibody target on their surface, for example in ...
For example, MAbs produced in a rat myeloma cell line (YB2/0) exhibit higher ADCC activity than MAbs produced in Chinese hamster ovary cells (CHOs) or in NSO myeloma cells. This is because YB2/0 cells produce MAbs witha lower fucose content. This finding led to the development of a CHO...
Antibody-dependent cell-mediated cytotoxicity (ADCC)Autoimmune thyroid diseaseFucosylationGlycoproteins*GlycosylationIgGImmunology*Patient cohortsThyroid peroxidase antibodies (TPOAb)Autoimmune thyroid diseases (AITD) are the most common group of autoimmune diseases, associated with lymphocyte infiltration and the ...
The extent of afucosylation, which refers to the absence of core fucose on Fc glycans, can correlate positively with the antibody‐dependent cellular cytotoxicity (ADCC) activity of a monoclonal antibody (mAb). Therefore, it is important to maintain consistent afucosylation during cell culture ...