aTables: Tables should be numbered consecutively and include a clear descriptive caption at the top. Avoid the use of structural formulas in the body of tables. Table footnotes should be given a footnote symbol as explained in the Footnotes section, proceeding by row rather than by column order...
(ng/mL)图S5CEA的测定校正曲线FigureS5CalibrationcurvesforCEAdetermination表S1流动注射化学发光法同时检测AFP和CEATableS1DetailsofflowinjectionchemiluminescentdetectionofAFPandCEA步骤操作1打开两个流路开关,用磷酸盐缓冲液清洗流通池,冲洗未结合的免疫试剂2关闭检测CEA的流路开关,打开检测AFP的流路开关,通入化学发光...
Next, 20 mL of 50 mM phosphate buffer (pH 6.5) was added in 6.0 mg of substrate (p-nitrophenyl-α-D-glucopyranose; Sigma-Aldrich, N1377-5G) and allowed to incubate at room temperature for 15 minutes. After incubation, the reaction was ceased by adding 50 μL of 2M glycine (pH 10)...
Table 1. Influence of cholesterol (CH)/sphingomyelin (SM) molar ratio, different phospholipids and steroids on the proportion of membrane domains, order parameter (S), correlation time (tc) and polarity correction factor (Pa) in sonicated lipid vesicles. Vesicles were labelled with MeFASL(2,11...
The DMAP compound with purity≥98% was obtained from Sigma Aldrich chemicals company, USA, and used as such for spectral analyses. The FTIR spectrum was recorded in the range 4000−400 cm−1 on the Bruker Tensor-37 spectrometer at 2 cm−1 spectral resolution using the KBr pellet techniq...
The N2-BET specific The protein used here was the bovine serum albumin (BSA) (fraction V, purchased from Sigma-Aldrich), which has an isoelectric point at pH 4.9–5.144,79 and a molecular weight of ≈ 66.5 kDa. All chemical reagents were of analytical grade, and Milli-Q water ...
Table 2. Thermal unfolding temperatures of Hsp90α, Hsp90β, Hsp90β + ATPγS and Hsp90β + radicicol monitored by different spectral parameters of FTIR spectra. Protein sampleAmide I′ band Tm (°C)Residual Amide II band TD1/2 (°C)Aggregation TA (°C)W1/2H Tm (°C)W1/2H Onset...
Sigma-delta approach Asynchronous interferogram sampling Spectrum One / 100 Optics Paraboloid 60 Deg Off Axis f = 40mm Flat Mirror Paraboloid 80 Deg Off Axis f = 100mm Ellipsoid 90 Deg Off Axis 1.2:1 Ellipsoid 45 Deg Off Axis 6:1 Sample Area Source J-Stop Image Interferometer Detector 红外...
(Alabaster, AL, USA) and most of the other chemicals were from Sigma-Aldrich (St. Louis, MO, USA). The N-terminal decapeptide of human pancreatic PLA2 containing a C-terminal thioester group (Ala-Val-Trp-Gln-Phe-Arg-Lys-Met-Ile-Lys- COSCH2COOH) was synthesized by SynPep Corp. (...
Spectrum IV has been chosen as the mostly suitable for such analysis (see Table 1 for the experimental details). As the first step of the analysis, the individual line intensities of 61 unsaturated not very weak and not blended lines (Jmax.=9 and Kamax.=8) have been determined from the ...