A Saccharomyces recombinase system that can be expressed also in Drosophila . FLP is a recombinase and FRT defines target sites of transposable elements (see Fig. F30 ). The system is very similar to the Cre-loxP of bacteriophage P1. The 43-kDa "flip" (FLP) is encoded by the 2渭 ...
关键词:FLP/FRT系统,位点特异性重组,转基因,高等真核生物 Abstract:FLP/FRT site-specific recombination system derived from 2 μm plasmid of yeast has been widely used in Arabidopsis thaliana, Oryza sativa Linnaeus, Mus musculus, Drosophila melanogaster, Caenorhabditis elegans and other higher eukaryotic ...
Ecole Normale Supérieure de Lyon. The Tomato/GFP-FLP/FRT method involves visualizing mosaic photoreceptor cells in living Drosophila. It can be used to follow individual photoreceptor cell fates in the retina for days or weeks. This method is ideal for
We describe here the Tomato/GFP-FLP/FRT method, which can be used for rapid clonal analysis in the eye of living adult Drosophila. Fluorescent photoreceptor cells are imaged with the cornea neutralization technique, on retinas with mosaic clones generated by flipase-mediated recombination. This ...
A Saccharomyces recombinase system that can be expressed also in Drosophila . FLP is a recombinase and FRT defines target sites of transposable elements (see Fig. F30 ). The system is very similar to the Cre-loxP of bacteriophage P1. The 43-kDa "flip" (FLP) is encoded by the 2μ circu...
FLP/FRT site-specific recombination system derived from 2 μm plasmid of yeast has been widely used in Arabidopsis thaliana, Oryza sativa Linnaeus, Mus musculus, Drosophila melanogaster, Caenorhabditis elegans and other higher eukaryotic organisms, and gradually become one of the powerful tools of gene...
FLP/FRT site-specific recombination system derived from 2 μm plasmid of yeast has been widely used in Arabidopsis thaliana , Oryza sativa Linnaeus, Mus musculus , Drosophila melanogaster , Caenorhabditis elegans and other higher eukaryotic organisms, and gradually become one of the powerful tools of...
The FLP recombinase of yeast catalyzes site-specific recombination in the Drosophila genome Cell (1989) N. Hasan et al. Escherichia coli genome targeting, I. Cre-lox-mediated in vitro generation of ori− plasmids and their in vivo chromosomal integration and retrieval Gene (1994) D. Kalderon ...
(InstituteofSericultureandSystemsBiology,SouthwestUniversity/KeySericulturalLaboratoryoftheMinistryofAgriculture, Chongqing400716) Abstract:FLP/FRTsite-specificrecombinationsystemderivedfrom2μmplasmidofyeasthasbeenwidelyusedin Arabidopsisthaliana,OryzasativaLinnaeus,Musmusculus,Drosophilamelanogaster,Caenorhabditiselegansand...
The FLP/FRT system permits rapid phenotypic screening of homozygous lethal mutations in the context of a viable mosaic fly. Combining this system with ovoD dominant female-sterile transgenes enables efficient production of embryos derived from mutant germline clones lacking maternal contribution from a ...