位点特异性重组(site-specific recombination)技术是近 20 年来发展起来的一项重要的分子生物学技术,该系统主要是通过借助能够识别特异性位点的重组酶(site-specific recombinase,SSR),在基因组上对外源 DN…
Produce of marker-free transgenic tobacco plants by FLP/frt recombination system. Chinese Journal of Biotechnology, 2006, 22(5): 744-750. (in Chinese)单晓映,李蓓,张举仁. 利用FLP/frt重组系统产生无选择标记的转基因烟草植株[J].生物工程学报,2006,(05):744-750.doi:10.3321/j.issn:1000-3061.2006....
位点特异性重组(site-specific recombination)技术是近 20 年来发展起来的一项重要的分子生物学技术,该系统主要是通过借助能够识别特异性位点的重组酶(site-specific recombinase,SSR),在基因组上对外源 DNA 进行操作,可以实现基因的置换、倒置和删除等。FLP/FRT 系统就属于位点特异性重组系统的一种。 FLP是一个重组酶...
1) flp-FRT recombination system flp-FRT重组系统2) FLP/frt system FLP/frt系统3) FLP-FRT site-specific recombination FLP-FRT位点特异性DNA重组4) FLP recombinase FLP重组酶 1. The plasmids containing different copies of SA 28 gene expression cassette were integrated into different chromosomal ...
Progress in Research of FLP/FRT Site-Specific Recombination System in Higher Eukaryotes ZHAO Ai-Chun, LONG Ding-Pei, TAN Bing, XU Long-Xia, XIANG Zhong-Huai PDF 6125 被引次数 17 摘要/Abstract 摘要:来源于酵母2 μm质粒的FLP/FRT位点特异性重组系统已经被广泛应用于拟南芥、水稻、小鼠、果蝇、线虫...
(flippaserecombinationenzyme).FLP是特异性重组酶,作用的靶序列为2μm双链环的2个反向重复序列中包含有3个13bp的FLP重组酶结合区和1个8bp的含限制性酶切位点的非对称间隔区的共48bp的FRT位点(FLPrecombinationtarget).自1993年Lyznik等[11]利用FLP/FRT系统瞬时转化玉米和水稻的原生质体以来,FLP/FRT系统已被广泛...
关键词:FLP/FRT 系统;位点特异性重组;转基因;高等真核生物 Progress in Research of FLP/FRT Site-Specific Recombination System in Higher Eukaryotes ZHAO Ai-chun, LONG Ding-pei, TAN Bing, XU Long-xia, XIANG Zhong-huai (Institute of Sericulture and Systems Biology, Southwest University/Key Sericultur...
A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked ... T Hoang,R Karkhoff-Schweizer,A Kutchma,... - 《Gene》 被引量: 0发表: 1998年 Site-specific deletions of chromosomally located DNA segmen...
Recombination (X) takes place within the core sequence and between FLP binding sites. Figure F30. FLP/FRT This, as well as the Cre/loxP system, can be used for site-specific integration of transgenes and also for engineering chromosomal rearrangements in higher eukaryotes. recombinase , Cre/...
In prokaryotes, few studies have applied the flippase ( FLP )/P1-flippase recombination target ( LoxP-FRT ) recombination system to switch gene expression. This study developed a new method for switching gene expression by constructing an FLP / LoxP-FRT