FLP/FRT 系统就属于位点特异性重组系统的一种。 FLP是一个重组酶(flippase,Flp),类似于Cre,它识别的位点是FRT,当FRT在同一条DNA上且方向相同时,则两FRT位点之间的基因则被删除;FRT方向相反,则基因被倒转。FRT:short flippase recognition target (FRT)。 Red recombination system中的质粒pCP20(42°培养可消除)...
Definition The FLP/FRT system is a yeast site‐specific recombination system applicable to many model organisms. The FLP/FRT system has been used in Drosophila and several other organisms to induce somatic recombination, generate chromosomal rearrangements, and as a basis for gene targeting. To ...
Flp-In™ System The Flp-In™ System allows for stable integration and expression of your gene of interest to deliver single-copy isogenic cell lines. Flp-In™ expression involves introduction of a Flp Recombination Target (FRT) site into the genome of ...
InvitrogenÆs Flp-In Complete System allows integration and expression of your gene of interest in mammalian cells at a specific genomic location. The Flp-In System involves introduction of a Flp Recombination Target (FRT) site into the genome
1. 一旦成功构建含FRT整合位点的Flp-In母细胞株,接下来构建表达目的基因稳定细胞株的工作就快速、高效; 2. Flp-In系统能构建同基因型的稳定细胞株; 3. Flp-In系统可以是多克隆稳定细胞株,无需纯化。 Flp-In™ System描述: Flp-In系统依据酿酒酵母的DNA重组系统的特点,高效构建稳定哺乳动物表达细胞株。这种DNA...
关高等真核模式生物中的研究现状,讨论了FLP/FRT系统在研究中存在的主要问题、应用前景和发展方向。 关键词:FLP/FRT系统;位点特异性重组;转基因;高等真核生物 ProgressinResearchofFLP/FRTSite-Specific RecombinationSysteminHigherEukaryotes ZHAOAi-chun,LONGDing-pei,TANBing,XULong-xia,XIANGZhong-huai (InstituteofSer...
关键词: FLP/FRT系统, 位点特异性重组, 转基因, 高等真核生物 Abstract: FLP/FRT site-specific recombination system derived from 2 μm plasmid of yeast has been widely used in Arabidopsis thaliana, Oryza sativa Linnaeus, Mus musculus, Drosophila melanogaster, Caenorhabditis elegans and other higher ...
2) flp-FRT recombination system flp-FRT重组系统 3) FLP-FRT site-specific recombination FLP-FRT位点特异性DNA重组 4) FRT-relation FRT关系 1. The connections between theFRT-relationand the compatibility condition are discussed. 讨论了 FRT关系与相容性关系的联系 ,并指出这样的相容性关系是由FRT关系所...
flp-FRT重组系统2) FLP/frt system FLP/frt系统3) FLP-FRT site-specific recombination FLP-FRT位点特异性DNA重组4) FLP recombinase FLP重组酶 1. The plasmids containing different copies of SA 28 gene expression cassette were integrated into different chromosomal loci via FLP recombinase mediated ...
5) site-specific recombination system 位点特异性重组系统 1. Construction of FLP/frtsite-specific recombination systemfor monocotyledonous plants; 单子叶植物的FLP/frt位点特异性重组系统的构建 更多例句>> 6) site-specific recombination 位点特异性重组技术 ...