In view to the Fmoc-deprotection reaction, basicity (pK_a-value) is necessary to form the dibenzofulvene (DBF). The nucleophilicity of the reagent is important to build up the Fluorenylmethyl-base adduct (Fm-base adduct). Stronger deprotection reagents like DBU and DBN with a higher pK_a ...
Usually I run it with 20% piperidine in DMF but I am afraid about a possible reaction between the azido group and the free amino group after deprotection. I mean, which influence could have piperidine after the Fmoc deprotection in a possible reaction between azide and amino group (piperidine ...
bThe reaction time and deprotection mixture is described in Chapter 3, Section 10. 1.2.5 Cleavage of peptide from the resin using gaseous ammonia The cleavage procedure for removal of the peptide from the resin as the amide will differ slightly depending on the nature of the solid support. ...
Re: FMOC deprotection and work-up «Reply #8 on:March 04, 2022, 12:07:02 PM » No, they are quite similar, though DCM is a bit more polar. I seldom have met small molecules where it dissolved in one but not the other.
图1 纳米K2CO3脱保护的通用反应路线Fig.1 General deprotection reaction route using nano-K2CO3 1.2.3 目标产物的结构表征 L-Leu-OBn·HCl:依据通法,脱除Fmoc-L-Leu-OBn中的保护基Fmoc,处理后得到白色固体L-Leu-OBn·HCl,收率为100%。1H-NMR(500 MHz,D2O,298 K) δ: 7.20～7.19(2H,d,J=5.0 Hz,—...
Fmoc Amino Acid Deprotection The Fmoc group of Fmoc amino acids is acid stable but is removed with a base through a β-elimination reaction. The Fmoc group of Fmoc amino acids is less labile to primary amines than secondary amines, thus minimizing premature Fmoc deprotection by the N-terminal...
The Boc group on the side chain is stable under basic conditions and remains in place even through many Fmoc deprotection cycles. The Boc group of Fmoc-Lys(Boc)-OH is readily removed with trifluoroacetic acid (TFA) during cleavage of the peptide product from Wang resin or Rink amide resin....
Fmoc-Trp(Boc)-OH is widely used in the pharmaceutical and biotechnology industries for the production of peptide-based drugs and research peptides. Its stability, compatibility with solid-phase synthesis techniques, and ease of deprotection make it a valuable tool in peptide chemistry. View more+ ...
somewhat soluble in NMP or DMF. The trityl group is removed with TFA. This reaction is usually complete within 1 hour at room temperature, but is slower when the Asn(Trt) residue in at the N-terminal of the peptide. In these cases, the deprotection reaction should be extended to 2 ...
Base-induced side reactions in Fmoc-solid phase peptide synthesis: Minimization by use of piperazine as Nα-deprotection reagent Base-induced aspartimide (cyclic imide) and subsequent base adduct formation in the Fmoc-solid phase synthesis of sensitive sequences are serious side reac... JD Wade,...