mitogen‐activated protein kinase (MAPK) signalingRskActivation signals from bacterial stimuli set into motion a series of events that alter the abbreviated lifespan of neutrophils. These studies show that the bacterial chemoattractant, formyl-Met-Leu-Phe (fMLP), promotes the phosphorylation/inactivation...
硕士学位论文 entry,Ca2+influxispredominantlymediatedbystore.operatedCa2+entry(SOCE)in蹦LP.stimulatedhumanneutrophils.TherearegoodevidencethatOrailandSTIMlaretwoessentialmolecullarscomprisingSOCEentitytobeinvolvedinneutrophilpolarizationandNADPHproducts,howevertheroleoftransientreceptorpotentialcanonicalI(TRPC1),asCa2+.pe...
In a uniform concentration of fMLP, neutrophils start to polarize with distinctive phenotypes, such as a dramatic increase in cell area, adopting an elongated shape, and formation of polarized signaling molecules and sub-cellular structures within the cell. TIR-FM microscopy uses a thin evanescent ...
The number of expressed by peripheral blood neutrophils taken from healthy individuals was quantitated using a direct fluorescence label analysed on the FACScan. Receptor numbers, assayed on cells from heparinised blood prepared at 4 degrees C or 37 degrees C or in the presence of LPS (30 ng/...
7.Isolation of human neutrophils from venous blood.[J].William M;Nauseef,Methods in molecular biology (Clifton, N.J.).2007,第期 8.MCP-1-stimulated chemotaxis of monocytic and endothelial cells is dependent on activation of different signaling cascades.[J].Kukhtina NB;Arefieva TI;...
The final preparation thus obtained contained more than 95% neutrophils, as estimated by differentially counting 200 Giemsa stained cells under the microscope. Show abstract Splitomicin inhibits fMLP-induced superoxide anion production in human neutrophils by activate cAMP/PKA signaling inhibition of ERK ...
The pathway pair 'antigen presentation pathway' and 'fMLP signaling in neutrophils' got the best AUC value of 1.000, which indicated that this pathway cross-talk could distinguish AS patients from the normal subjects. Predicting pathway cross-talks in ankylosing spondylitis through investigating the in...
Here, we investigated whether phospholipase D (PLD), a signaling molecule linked to chemoattractant activation of neutrophils, is necessary for both Mac-1-dependent and Mac-1-independent migration. Both fMLP and C5a increased neutrophil expression of the Mac-1 activation epitope, indicating PLD was...
Using short- and long-term in vitro assays, as well as an in vivo chemotaxis assay, we investigated the importance of PI3K in response to the prototypic chemoattractant fMLP. Exposure of neutrophils to fMLP induced an immediate polarization, which resulted in directional migration towards fMLP ...
Large inhibition against the OZ-induced production of O 2 - was only observed in neutrophils treated with GF109203X, a PKC inhibitor. The present study indicates that receptor dependence exists in the ERK1/2 signaling pathway leading to the activation of NADPH oxidase.Waki...