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The GFP-based superecliptic pHluorin (SEP) enables detection of exocytosis and endocytosis, but its performance has not been duplicated in red fluorescent protein scaffolds. Here we describe “semisynthetic” pH-sensitive protein conjugates with organic
The present invention relates to a novel near-infrared fluorescent probe having specificity for mitochondria due to a hydrophobic group attached to a silicon-rhodamine core, and the
It was interesting to see that HPsensor 2 showed decreased signal with ApoMb and the least signal with Mb after exposure to UV light (Supplementary Fig. 18) which is in line with the fluorescence data. Discussion TsehnesinnogvpelroBtOeinDIhPyYdrdoypehsowbiictihtyaraynlds2u-bmsteittuhtoixoy...
Using dual-color TIRF excitation at 488 and 568 nm and dual-color emission detection, Ca2+ transients evoked by blue light (458 nm, 5 s, 13.6 mW/mm2) were detected in astrocytes expressing the light-gated Ca2+-translocating CatCh-YFP and loaded with CaRuby-Me (5 μM, 10 min) [41]....
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Materials All of the solvents used in this work were of analytical purity, and the materials required were obtained from commercial suppliers or prepared by our laboratory without further purification of the commercially supplied chemicals before use. Deionized water was used for all measurements. All...