The rated light output of some CFLs is based on the bulb being upside down (i.e. screw base at top), but sometimes in a sealed fixture that actually decreases the life of the bulb because heat rises to the ballast area causing over heating. If the screw base is at the bottom, the ...
Hence, it is prudent to gain insights into recent advancements in nanoscopic imaging of mitochondria using both FPs as well as chemical probes in the light of contemporary literature. Therefore, this section mainly summarizes the design, synthesis, and utility of various fluorescent probes (along ...
Chromophore-assisted light inactivation (CALI) can inactivate proteins or organelles by exciting fluorophores attached to them that locally generate damaging reactive superoxide. Historically, antibodies were used to direct a photosensitizer to its target, but the fusion of fluorescent proteins or SNAP-tags...
Coupling BAPTA to seminaphtofluorescein (an ‘extended fluorescein’ variant) produces a visible-light excited long-wavelength emitting Ca2+ indicator that has a Ca2+-dependent extinction near 488 nm and fluorescence emission with peaks at 650 and 525 nm. Because the largest fluorescence change occur...
2.2.6. Fluorescence Imaging Experiments Imaging was carried out with a confocal laser fluorescence inverted microscope using a Spectral Confocal Microscopy TCS SP5 II Leica system equipped with a white light laser (470–670 nm) and a 405 laser, and an environmental cell culture chamber that ...
Combining its favorable light-up fluorescence feature, high selectivity, long wavelength emission, large Stokes shift, low cytotoxicity, and good membrane permeability, we applied NQ-DCP to achieve the real-time visualization of hNQO1 in live cells and mice. The characteristic target-to-background ...
The CD solution when kept under UV light (365 nm) exhibited a green color, but in daylight, it was of a brownish yellow color (Figure 1B). The fluorescence spectra of the CDs are presented in Figure 2A. The fluorescence emission spectra showed strong emission, with the highest intensity ...
[16]. For this method, when the UV light reach a fluorophore, it causes it to go into an excitation state and makes it undergo conformational changes (partly dissipating some of this energy); when the fluorophore returns to the normal state, a light at a different wavelength is emitted [...
A longer-wavelength light than 800 nm was not available due to the limitations of the fluorospectrophotometer used in this study, but a longer wavelength source, such as a near-IR laser, might induce a higher PL intensity. A 790 nm light stimulated the 480 nm up-conversion emission, and ...