In addition, the absorbance of the short-wavelength light by the biological samples will also limit the application of the probes in deep sample penetration imaging. The satisfying wavelength for in vivo imaging is in the near-infrared range (NIR, 650–950 nm), which can avoid interference ...
First we replaced the big fluorescent light near our stove. It was incredibly simple to do so we’ve outlined a step-by-step process below: Step 1: Turn Off The Power Whenever you’re working with a light fixture, it’s always good to turn off the power to this light at the circuit...
Fluorescent tubes have such a long life and are so inexpensive (with the exception of some of the "natural light" bulbs) that it makes no sense to skimp. I acknowledge that replacing all the bulbs is not the most economical solution...it's just a practical viewpoint from someone (me) ...
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3. Thanks to its light shield, the ZOE Fluorescent Cell Imager can be used in ambient light; darkroom operation is not required. However, it is not designed to be operated in direct sunlight. 4. Insert the supplied power cord into the instrument with its flat side facing down and without...
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SNAP tags that allow labelling with fluorescent dyes and targeted chromophore-assisted light inactivation; and nanobodies targeted to lumenal sub-compartments of the secretory pathway. We also developed two methods for crosslinking tagged proteins: a dimeric nanobody, and RFP-targeting and GFP-targeting...
Coupling BAPTA to seminaphtofluorescein (an ‘extended fluorescein’ variant) produces a visible-light excited long-wavelength emitting Ca2+ indicator that has a Ca2+-dependent extinction near 488 nm and fluorescence emission with peaks at 650 and 525 nm. Because the largest fluorescence change occur...
Fluorescence spectra were taken on a FL3C-111 TCSPC luminescence spectrometer equipped with a 20-kW xenon discharge lamp as a light source (Horiba, Kyoto, Japan). X-ray powder diffraction (XRD) spectra were taken on a Rigaku D/MAX2500V X-ray diffractometer (Rigaku, Tokyo, Japan). The mic...
2.2.6. Fluorescence Imaging Experiments Imaging was carried out with a confocal laser fluorescence inverted microscope using a Spectral Confocal Microscopy TCS SP5 II Leica system equipped with a white light laser (470–670 nm) and a 405 laser, and an environmental cell culture chamber that ...