Flow-through purificationDepth filtrationBiopharmaceutical processA depth filter is widely used in biopharmaceutical manufacturing process. In this study, we found that buffer exchange to reduce conductivity dramatically improved the removal of impurities in depth filtration. The host cell protein clearance ...
The porous monoliths were synthesized within silica capillaries from glycidyl methacrylate and ethoxylated trimethylolpropane triacrylate precursors, providing a tortuous pore structure with high surface area for the immobilization of antibodies or other biosensing ligands. The unique morphology of the monolith ...
The disposable immunoelectrode consists of highly dispersed carbon modified by immobilized anti-protein A antibodies. The flow-through technique permits high automation of the entire measuring procedure. The detection is based on peroxidase-labeled anti-IgG antibodies captured by the immunoelectrode as a...
Table 1 Antibodies used for immunocytochemical and immunohistochemical staining Full size table To confirm endothelial cell identity, surface marker expression of the isolated cells was detected via immunocytochemical staining. To this end, 1 × 105 cells per 1.7 cm2 chamber were grown on gelatin...
多通道流动电泳 (MFE)是一种制备型流动电泳技术 ,采用计算流体力学方法描述MFE设备中的流体流动特性 ,以指导分离过程和分离设备设计 。 4) counter immunoelectrophoresis 对流免疫电泳法 1. Methods: Immunoblotting technique (IBT) andcounter immunoelectrophoresis(CIE) were used to detect antibodies against polypep...
Electrochemical flow-through biosensorAnti-DNA autoantibodyHuman serumAnti-DNA antibodies in human sera are an important diagnostic indicator of systemic lupus ... RL Rubin,D Wall,KN Konstantinov - 《Biosensors & Bioelectronics》 被引量: 10发表: 2014年 Acridine orange-induced signal enhancement effect...
A flow-through immunosensor based on a high-surface-area carbon immunoelectrode has been developed. Dispersed carbon material serves as a carrier for immobilized antibodies and at the same time as an electrode material. The 'sandwich' scheme of immunoassay has been used. Iodine formed as a result...
Flow-through chromatography is perhaps one of the most common used tools to polish monoclonal antibodies. Operating close to neutral pH and at low conductivity, many viruses and trace impurities such as DNA, endotoxin and a large percentage of host cell proteins display a negative charge, thus bi...
individual antibodies may be sensed electrically when they bind a peptide epitope tethered to electrodes. Where no binding occurs, the signal will be zero, with no electrical background leakage in the circuit, in contrast to the commonly-used fluorescence (ELISA) assays which suffer from unwanted ...
(2012), other scientists have successfully tested STMAs for different tasks such as purification of monoclonal antibodies (Kang et al., 2013) or in viral clearance studies for removal of bacteriophages or minute virus of mouse (Faber et al., 2009,b; Riordan et al., 2009a,b). In this ...