Floral dip: a simplified method for Agrobacterium -mediated transformation of Arabidopsis thalianaThe Agrobacterium vacuum infiltration method has made it possible to transform Arabidopsis thaliana without plant tissue culture or regeneration. In the present study, this method was evaluated and a ...
Arabidopsis thaliana floral dip transformation method. Arabidopsis floral dip transformation is notable for a number of reasons. First, it is strikingly simple to perform. Agrobacterium is applied to flowering ... A Bent - 《Methods in Molecular Biology》 被引量: 238发表: 2006年 Improving transform...
系统标签: floral dip 拟南芥 tumefaciens arabidopsis agrobacterium FloralDip9191From:MethodsinMolecularBiology,vol.286:TransgenicPlants:MethodsandProtocolsEditedby:L.Peña©HumanaPressInc.,Totowa,NJ6FloralDipAgrobacterium-MediatedGermLineTransformationStevenJ.CloughSummaryManyresearchersusethefloweringplantArabidopsisth...
As a simple and high-efficient genetic transformation approach, floral dip method has been widely used for the studies of gene function in IArabidopsis/I and other plant species. However, there is no report about its applications in maize. In this study, the floral-dip method of transformation...
This is a higher transformation rate than those reported for Arabidopsis thaliana and other plant species, using floral-dip transformation. It is also the highest, which has been reported so far, for flax transformation using other methods for transformation. 展开 ...
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana Plant J., 16 (1998), pp. 735-743 Google Scholar 36 U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature, 227 (1970), pp. 680-685 Google Scholar...
floral dipA method is described for the floral transformation of wheat using a protocol similar to the floral dip of Arabidopsis. This method does not employ tissue culture of dissected embryos, but instead...doi:10.1007/978-1-59745-379-0_6Sujata Agarwal...
Floral dip a simplified method for Agrobacterium-mediated 下载积分:700 内容提示: The Plant Journal (1998) 16(6), 735–743TECHNICAL ADVANCEFloral dip: a simpli ed method for Agrobacterium-mediatedtransformation of Arabidopsis thalianaSteven J. Clough and Andrew F. Bent*Department of Crop Sciences, ...
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana Plant Journal, 16 (1998), pp. 735-743 View in ScopusGoogle Scholar Coen et al., 1990 E.S. Coen, J.M. Romero, S. Doyle, R. Elliott, G. Murphy, R. Carpenter floricaula: A homeotic gene ...
thaliana sbt1.8 mutant plants by the floral dip method. Transgenic plants were selected in soil by spraying with Basta (Basta:H2O = 1:1000). In total 13 independent T1 transgenic lines were used for phenotype observation. In situ hybridization and RT-qPCR For pineapple stage 4 ovules and...