Das P, Joshi N (2011) Minor modifications in obtainable Arabidopsis floral dip method enhances transformation efficiency and production of homozygous transgenic lines harboring a single copy of transgene. Adv Biosci Biotechnol 2:59–67Das, P., and Joshi, N.C. 2011. Minor modifications in ...
Floral dip: a simplified method for Agrobacterium -mediated transformation of Arabidopsis thalianaThe Agrobacterium vacuum infiltration method has made it possible to transform Arabidopsis thaliana without plant tissue culture or regeneration. In the present study, this method was evaluated and a ...
In the model flowering plant Arabidopsis thaliana, the floral-dip transformation method has replaced all previous methods because of its simplicity, efficiency, and low cost. Specifically, shoots of young flowering Arabidopsis plants are dipped in a solution of Agrobacterium tumefaciens carrying specific ...
Arabidopsis floral dip transformation is notable for a number of reasons. First, it is strikingly simple to perform. Agrobacterium is applied to flowering Arabidopsis plants that subsequently set seed, and transgenic plants are then selected among the progeny seedlings. Because no plant tissue culture...
Many researchers have developed various methods for in-planta or floral dip transformation of Arabidopsis thaliana, one of the simple protocol and widely used to produce transgenic Arabidopsis. As the efficiency and ease of getting a transformant is very much time consuming effort and less number of...
F. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 16, 735–743 (1998). Article CAS PubMed Google Scholar Nakamura, Y. et al. Digalactosyldiacylglycerol is a major glycolipid in floral organs of Petunia hybrida. Lipids 38, 1107–...
Ghedira R.;et al.(2013) The efficiency ofArabidopsis thalianafloral dip transformation is determined not only by theAgrobacteriumstrain used but also by the physiology and the ecotype of the dipped plant.Moecular Plant Microbe Interaction, 26(7), 823-832. ...
F. Floral dip: a simplified method for agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 16, 735–743 (1998). Article CAS PubMed Google Scholar Sandmann, M., Fuchs, J. & Lermontova, I. Immunolabeling of nuclei/chromosomes in Arabidopsis thaliana. Methods Mol. Biol. ...
F. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 16, 735–743 (1998). Article CAS Google Scholar Park, J., Lim, K., Kim, J.-S. & Bae, S. Cas-analyzer: an online tool for assessing genome editing results using NGS data....
F. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 16, 735–743 (1998). Article CAS Google Scholar Gibson, D. G. et al. Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat. Methods 6, 343–345 (2009). ...