Floral dip: a simplified method for Agrobacterium -mediated transformation of Arabidopsis thalianaThe Agrobacterium vacuum infiltration method has made it possible to transform Arabidopsis thaliana without plant tissue culture or regeneration. In the present study, this method was evaluated and a ...
The efficiency of Arabidopsis thaliana floral dip transformation is determined not only by the Agrobacterium strain used but also by the physiology and the... To evaluate the chromosomal background of different Agrobacterium strains on floral dip transformation frequency, eight wild-type Agrobacterium str...
F. Floral dip: a simplified method for Agrobacterium- mediated transformation of Arabidopsis thaliana. Plant J. 16, 735–743 (1998). 63. Murashige, T. & Skoog, F. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol. Plant 15, 473–497 (1962). 64. ...
Escherichia coli(E. coli) andAgrobacterium tumefaciensclones were grown on Luria-Bertani (LB) medium containing antibiotics as prescribed by plasmid-borne antibiotic resistance markers. Antibiotics were purchased from GoldBio.Pseudomonas syringaepv.tomatoDC3000 (PstDC3000) strains were grown as previously ...
Interestingly, most of the Arabidopsis mutants that are resistant to Agrobacterium transformation identified by root explant assays remain highly transformable by floral dip transformation [14]. The mechanisms and plant factors involved in Agrobacterium- mediated transformation may differ between wounded and ...
37. Clough SJ, Bent AF: Floral dip: a simplified method for Agrobacterium- mediated transformation of Arabidopsis thaliana. Plant J 1998, 16(6):735-743. 38. Wydro M, Kozubek E, Lehmann P: Optimization of transient Agrobacterium-mediated gene expression system in leaves of Nicotiana benthamiana...
Agrobacterium strains carrying the constructs containing putative interacting proteins were mixed and co-infiltrated in the abaxial surface of four-week-old Nicotiana benthamiana leaves at a final OD600 = 0.5 each. To enhance expression of fusion proteins, Agrobacterium C58C1 carrying the pCH32 helper...
The resulting pGWB2-GhPMEI53 Ti plasmid was introduced into Agrobacterium strains LBA4404 and GV3101. Transgenic cotton and Arabidopsis plants were generated through the callus induction method, using hypocotyl segments as explants and the floral dip method for Arabidopsis (Zhang et al. 2006; Ge ...
the expression ofAtALA7inArabidopsisand tobacco significantly increased the resistance to different strains ofV. dahliae, whereas the exogenous overexpression ofAtALA1in maize significantly increased the resistance to DON and ear rot disease, thus largely reducing the DON concentration in seeds. Our data...
All Agrobacterium strains were grown for 2 days, diluted to OD 1 in infiltration buffer and incubated for 2 h at room temperature before mixing in a 1:1 ratio with other strains and injecting. Three days after injection, interaction of the proteins was scored by screening lower epidermal ...