这两篇文章都于2022年5月30日发布在Nature Biotechnology上,提出了两种新的全长单细胞RNA测序方法,其中Smart-seq3xpress是基于近期的Smart-seq3进行的小型化和简化改进,能够减少试剂的使用并提高通量。而FLASH-seq则是基于Smart-seq2进行...
图1FLASH-seq技术方法概述 Smart-seq2 (SS2) (SS2),由使用模板转换逆转录酶(RT)的初始逆转录反应组成,如小鼠Moloney白血病病毒(MMLV)酶,该酶在cDNA的5 '端添加一个已知序列,然后通过聚合酶链反应 (PCR) 扩增全长 cDNA,最后片段化并添加测序接头,整个工作流程中,单个细胞在 PCR 板的不同孔中进行处理。作者对...
为此,研究人员开发出一种名为FLASH-seq的单细胞RNA测序新方法。与Smart-seq3相比,它具有更高的灵敏度和更短的手动操作时间。整个操作可在约4.5小时内完成,易于自动化,并且可以轻松缩小规模,以减少资源消耗。 他们指出,FLASH-seq方法与其他方法相比节省2-3.5小时,并能够在HEK293T细胞中检测到更多基因。这种灵敏度的...
We present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire FS protocol can be performed in ~4.5 hours, is simple to automate and can be easily miniaturized to decrease res...
We present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire FS protocol can be performed in ~4.5hours, is simple to automate and can be easily miniaturized to decrease ...
CTranslate2 Flash Attention regurgitates repeated tokens - seq2seq它应该适用于Onmt-py的旧版本或新...
Re: 下载程序报错:A fatal error occurred: Failed to write compressed data to flash after seq 5 (result was C100) Quote PostbyESP_@In逍遥子»Sat Jan 19, 2019 9:16 am 建议: 1.建议确保电源稳定、以及串口连接无异常 2.检查 flash 相关参数 ...
(device='cuda',dtype=torch.int32)flash_attn_with_kvcache(q=q,k_cache=k_cache,v_cache=v_cache,k=k,v=v,rotary_cos=None,rotary_sin=None,cache_seqlens=cache_seqlens,cache_batch_idx=cache_batch_idx,# softmax_scale=None,# causal=True,# window_size=(-1, -1),# rotary_interleaved=...
Platforms: rocm This test was disabled because it is failing in CI. See recent examples and the most recent trunk workflow logs. Over the past 3 hours, it has been determined flaky in 3 workflow(s) with 9 failures and 3 successes. Debugg...
A fatal error occurred: Failed to write compressed data to flash after seq 5 (result was C100) make: *** [/home/yangruining/esp/esp-idf/components/esptool_py/Makefile.projbuild:63:flash] 错误 2 1 post • Page1of1 Return to “ESP-IDF” ...