Here, we present three variants of the FS protocol.Standard FLASH-seq (FS), which builds upon Smart-seq2 developed in the past, is non-stranded and does not use unique molecular identifiers (UMIs) but still remains the easiest method to measure gene expression in a cell population.FLASH-...
We present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire FS protocol can be performed in ~4.5 hours, is simple to automate and can be
370.27 BogoMIPS (lpj=1851392) Mount-cache hash table entries: 512 Initializing cgroup subsys debug Initializing cgroup subsys cpuacct Initializing cgroup subsys freezer CPU: Testing write buffer coherency: ok net_namespace: 936 bytes NET: Registered protocol family 16 bio: create slab <bio-0> at...
Router(config)#class-map VOICE Router(config-cmap)#match protocol rtp Router(config-cmap)#exit Router(config)#policy-map COMPRESS Router(config-pmap)#class VOICE Router(config-pmap-c)#compression header ip rtp Router(config-pmap-c)#exit Router(config-pmap)#exit Router(config)#interface serial ...
Resource Reservation Protocol (RSVP) is an example of an IntServ approach to QoS. Because IntServ must be configured on every router along a packet’s path, the main drawback of IntServ is its lack of scalability. Differentiated Services (DiffServ)—DiffServ, as the name suggests, ...
RNA sequencingTranscriptomicsWe present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire FS protocol can be performed in ~4.5hours, is simple to automate and can be easily ...
Router(config)#class-map VOICE Router(config-cmap)#match protocol rtp Router(config-cmap)#exit Router(config)#policy-map COMPRESS Router(config-pmap)#class VOICE Router(config-pmap-c)#compression header ip rtp Router(config-pmap-c)#exit Router(config-pmap)#exit Router(config)#interface serial ...
Protocol (using NBAR) Traffic matching another class-map Incoming interface MAC address (source or destination) Range of UDP port numbers In the following example, you are matching traffic based on a variety of the preceding criteria: Router(config)#class-map match-any INT ...
Round robin queuing—This type of queuing places traffic into multiple queues, and packets are removed from these queues in a round-robin fashion, which avoids the protocol- starvation issue that PQ suffered from. Weighted Round Robin (WRR) queuing—This type of queuing can place a weight on ...
Resource Reservation Protocol (RSVP) is an example of an IntServ approach to QoS. Because IntServ must be configured on every router along a packet’s path, the main drawback of IntServ is its lack of scalability. Differentiated Services (DiffServ)—DiffServ, as the name suggests, ...