研究人员还比较了 RAGE 和拉曼激活细胞弹射(RACE-seq)两种技术对 FISH-scRACS-seq 的适用性。结果显示,FISH-scRACS-seq 在所有组装指标(基因组完整性、测序偏差和组装连续性)上均优于 RACE-seq,能恢复更多基因 ,因此后续实验选择 RAGE 用于 FISH-scRACS-seq 。此外,通过将 FISH-scRACS-seq 获得的 SAG 与 s...
Current assays fail to address breast cancer's complex biology and accurately predict treatment response. On a retrospective cohort of 1082 female breast tissues, we develop and validate mFISHseq, which integrates multiplexed RNA fluorescent in situ hybridization with RNA-sequencing, guided by laser ...
肿瘤FISH分析的假阴性结果通常是由于取样不良造成的,即送检的样本中很少或没有肿瘤细胞。然而,在本病例中,阴性结果可能是由于细胞遗传学隐匿性重排造成的,而商业FISH探针无法检测到这一情况。在罕见的白血病样本,甚至在更少的肉瘤病例中,由于异常染色体重排导致的类似假阴性FISH结果也有描述。 在本例中,FISH假阴性结果...
1例骨DLBCL-NOS FISH检出BCL2 重排,而 RNAseq 显示 CIITA-IGH 重排。1例FISH 和 RNAseq 未检出 MYC、BCL2 和 BCL6 重排的淋巴结 DLBCL-NOS 存在 EIF4E3-FOXP1 重排。1例FISH 和 RNAseq 未检出 MYC、BCL2 和 BCL6 重排的淋巴结 DLBCL-NOS 存在 SH3BP-METTL6 重排。 讨论 重现性细胞遗传学异常有助...
17, 18). ARTseq-FISH provides similar relative protein abundance compared to classic immunofluorescence (Supplementary Fig. 19). We determined a detection efficiency of ~47% (for Nanog mRNA) by comparing mRNA quantification using ARTseq-FISH with conventional single molecule RNA FISH (smFISH)27 (...
自己整理,测试完全可用。从直接下载数据到一般的出图 #!/usr/bin/bash #ly_20211215_atac-seqpepiline #从下载数据到分析出图的一般流程 ### start_time=$(date+%s) #必要索引文件 bt2_index=/home/data/ssy49/data/index/mm10 #一些物种可以直接从bowtie官网下载索引: http://bowtie-bio.sourceforge....
(MYH) proteins, while a small number of hybrid fibers can express more than one MYH. By snRNA-seq and FISH, we show that the majority of myonuclei within a myofiber are synchronized, coordinately expressing only one fastMyhisoform with a preferential panel of muscle-specific genes. ...
We need support for in-house frequencies inseqvars query. Describe the solution you'd like Implement this based on the output ofseqvars aggregate. Describe alternatives you've considered N/A Additional context https://github.com/bihealth/varfish-server-worker/blob/5f782aee705ba360b2b0e6e94761...
CNV-seq检测结果为常染色体非整倍体,建议( ) A. 终止妊娠 B. 对其父母进行染色体核型分析或FISH检测 C. 对其父母进行染色体核型分析 D. 建议对父母进行染色体核型分析或者FISH检测 E. 结合超声检查的结果进行综合判断 相关知识点: 试题来源: 解析 A
Here we introduce Fish the ChIPs (FC), a computational pipeline aimed at a broad public of users and designed to perform complete ChIP-Seq data analysis of an unlimited number of samples, thus increasing throughput, reproducibility and saving time. Results Starting from short read sequences, FC...