Current assays fail to address breast cancer's complex biology and accurately predict treatment response. On a retrospective cohort of 1082 female breast tissues, we develop and validate mFISHseq, which integrates multiplexed RNA fluorescent in situ hybridization with RNA-sequencing, guided by laser ...
其次,由于检测到的t(12;22)不是MLS的特征,因此使用EWSR1基因的BAP进行中期FISH分析来补充,这项分析揭示了12/12中期扩散中的分裂信号(图1B)。最后,将提取的RNA进行转录组测序(RNA-seq),发现EWSR1的第7号外显子与DDIT3的第2号外显子之间存在框内EWSR1::DDIT3融合转录本。 图1 讨论 MLS的分子特征为位于...
FISHseq showed either mixed or monospecies colonization on the EBV, including oropharyngeal bacterial flora, Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus spp., and Fusobacterium sp. On 5/10 EBV, FISHseq visualized biofilms, on 1/10 microbial microcolonies, o...
FISHseq provided an added value for diagnostics in 79/124 cases (63.7%) and potentially would have impacted therapy in 95/124 (76.6%) of cases. By adding data through direct visualization and characterization of microorganisms, FISHseq reduced the number of inconclusive cases by 86.0%. ...
(MYH) proteins, while a small number of hybrid fibers can express more than one MYH. By snRNA-seq and FISH, we show that the majority of myonuclei within a myofiber are synchronized, coordinately expressing only one fastMyhisoform with a preferential panel of muscle-specific genes. ...
Here we introduce Fish the ChIPs (FC), a computational pipeline aimed at a broad public of users and designed to perform complete ChIP-Seq data analysis of an unlimited number of samples, thus increasing throughput, reproducibility and saving time. Results Starting from short read sequences, FC...
SeqMask is a deep learning tensorflow2 model for ATT&CK techniques and tactics classification for cyber threat intelligence - MuscleFish/SeqMask
Describe the solution you'd like Adjust the logic and models as necessary to import the results of seqvar query jobs. UI integration is a follow-up ticket. Describe alternatives you've considered N/A Additional context
RNAseq和FISH的结果总结于表1和图1。总体而言,RNAseq识别了FISH鉴定的1/7 MYC(14%)、3/8 BCL2(38%)和4/8 BCL6(50%)易位(图1)。在RNAseq检测到的2例MYC重排中,伴侣基因均为IGH,其中一例先前FISH检测到,另一例由于IHC显示MYC蛋白染色水平较低,先前未进行FISH检测。后一例患者后续进行了MYC 断裂分离...
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