NGS,即下一代测序技术(Next-generation sequencing),又称高通量测序技术(High-throughput sequencing, HTS)、第二代测序。通过受检样本与对照的测序信号进行对比,计算出MET拷贝数变化。 2 什么是FISH检测 FISH,即荧光原位杂交技术。以荧光探针标记MET基因和7号染色体着丝粒数,计算...
[4] Sun B, Qiu T, Zeng X, Duan J, Bai H, Xu J, Li J, et al. Detection of MET Polysomy by Next-generation Sequencing and Its Clinical Relevance for MET Inhibitors. Cancer Res Commun. 2023 Apr 4;3(4):532-539. [5] Lin X, Zheng...
Li B, Zhu H. Case report: Identification of atypical mantle cell lymphoma with CCND3 rearrangement by next-generation sequencing. Front Oncol. 2023 Mar 29;13:1145376. doi: 10.3389/fonc.2023.1145376. PMID: 37064110; PMCID: PMC10090691.[...
此外,可能还有改善的空间解决我们工作的核心优化问题。 参考文献:Haoyun Lei,et al. Tumor heterogeneity assessed by sequencing and fluorescence in situ hybridization (FISH) data. BioRxiv. 29 Feb,2020. (转化医学网360zhyx.com)
这里需要简单补充一点基础知识,NGS,又名下一代测序(Next Generation Sequencing),是相对于传统的第一代测序(Sanger Sequencing)而言的。 艾德生物、燃石医学、世和基因都是基于第二代测序技术(NGS)的应用(可逆末端终止测序法),对于后台数据分析能力要求高,已经是成熟技术。艾德生物的测序仪合作公司是贝瑞基因(IIIumina...
Ruiz-Ruano FJ, Cuadrado Á, Montiel EE, Camacho JPM, López-León MD (2015) Next generation sequencing and FISH reveal uneven and nonrandom microsatellite distribution in two grasshopper genomes. Chromosoma 124(2):221–234Ruiz-Ruano FJ, Cuadrado A, Montiel EE, Camacho JPM, Lopez- Leon MD...
Type I markers, both microsatellites and SNPs, can easily be identified in libraries of expressed sequences, either through traditional EST library sequencing or through NGS transcript sequencing methods such as RNAseq. While linkage maps can have many uses, one of the most useful is in mapping ...
and molecular markers of sex differentiation in fish, by far not only the most diverse group of vertebrates in terms of number of species but also, specially, in terms of reproductive strategies and sex-determining mechanisms. The sequencing of the genome in new species will eventually help to ...
[4] Dai L, Zhang H, Chen W, Xia Y, Qin S, Shao Y, Li J, Miao Y, Li B, Zhu H. Case report: Identification of atypical mantle cell lymphoma with CCND3 rearrangement by next-generation sequencing. Front Oncol. 2023 Mar 29;13:1145376. doi: 10.3389/fonc.2023.1145376. PMID: 37064110...
(Qiagen). The resulting RNA was tested for quality on an Agilent Bioanalyzer and all samples met quality standard of RNA Integrity Number (RIN) > 8. mRNA-focused sequencing libraries were designed with Illumina TruSeq v3 kits and sequenced for 150 bp paired-end reads on an Ilumina Hi...