Quantitative and sensitive methods for the detection of pseudouridine (Ψ) have been lacking. Now, a method termed 2-bromoacrylamide-assisted cyclization sequencing (BACS) has been developed that enables the accurate quantification of Ψ stoichiometry, precise identification of Ψ positions and robust ...
Data considerations for Human Genome Sequencing Introduction Ribonucleic acid (RNA) are polymeric molecules consisting of nucleotides, essential in the coding, decoding, regulation, and expression of genes. In the central dogma of molecular biology, the ...
Because of these advantages, researchers have rapidly adopted RNA-Seq as a general method to address this complex landscape. There are now different techniques, from whole transcriptome sequencing to targeted RNA sequencing, that each have their advantages an...
The imminent release of tissue atlases combining multichannel microscopy with single-cell sequencing and other omics data from normal and diseased specimens creates an urgent need for data and metadata standards to guide data deposition, curation and release. We describe a Minimum Information about Highl...
Use Cases:Three use cases in the fields of oncology, cardiology and infection control will demonstrate the capabilities of the HiGHmed approach. Each of the use cases entails diverse challenges in terms of data protection, privacy and security, including clinical use of genome sequencing data (onco...
Developing human brain single-nucleus library preparation and sequencing 开发人类大脑单核库制备和测序 Para_01 单细胞核从冷冻胎儿脑组织中分离出来,根据已发表的方案42进行。 简而言之,组织在匀浆缓冲液(250 mM蔗糖,25 mM氯化钾,5 mM氯化镁,10 mM Tris缓冲液,pH 8.0,1 M 1,4-二硫苏糖醇,0.1% Triton ...
Cell-cell interactions are important for information exchange between different cells, which are the fundamental basis of many biological processes. Recent advances in single-cell RNA sequencing (scRNA-seq) enable the characterization of cell-cell interactions using computational methods. However, it is ...
High-Throughput Sequencing Single-molecule Assay CRISPR Sucrose Density Gradient Centrifugation Cross-linking Inverse PCR Microscopy Cryo-EM Two-photon Microscopy Super-resolution Microscopy Machine Learning Calcium Imaging Freeze Fracture Technique Cell and Tissue Culture ...
Mutation load can be determined by sequencing, such as whole exome sequencing, and can be a powerful predictor of a person’s risk for developing cancer. Cancer research There are two main areas of focus for cancer research: prevention and treatment. Understanding how cancer develops can ...
In contrast, PCR or sequencing errors will result in errors in only one strand. This method uniquely capitalizes on the redundant information stored in double-stranded DNA, thus overcoming technical limitations of prior methods utilizing data from only one of the two strands....