Identification of the rainbowfish in Lake Eacham using DNA sequencingdoi:10.1071/ZO12106Culum BrownYagiz AksoyHilal VarinliMichael GillingsCSIRO PUBLISHING
probabilities and rc by implication, are determined by a series of steps that start with cross-linking spatially adjacent loci using formaldehyde, chopping the chromatin into fragments using restriction enzymes, ligating the fragments with biotin, followed by sequence matching using deep-sequencing ...
For protein-coding genes, sequencing reads from every fish were mapped to a mRNA reference from rainbow trout genome44 and our transcriptome assembly46. The expression value of each transcript was calculated in terms of TPM (transcript per million), and DE mRNAs between gravid and sterile fish ...
In addition to whole genome sequencing, SNPs can be identified through genome re-sequencing or RNA-Seq projects. For instance, genome re-sequencing projects have identified more than 8.3 and 9.7 million putative SNPs in channel catfish [18] and Atlantic salmon [19] respectively. Large numbers of...
Transcriptome sequencing was performed on these tissues, and the activity of enzymes associated with hypoxic stress in liver tissue was examined. At different time points, DEGs from different tissues were analyzed for GO and KEGG enrichment analyses. Based on our results, the hypoxia regulation ...
The ballan wrasse (Labrus bergylta) belongs to a large teleost family containing more than 600 species showing several unique evolutionary traits such as lack of stomach and hermaphroditism. Agastric fish are found throughout the teleost phylogeny, in qu
The full-length of saMSTN-2gene sequence was amplified by PCR, using genomic DNA from fins of threeS. aurataindividuals: fish #17G (found following sequencing to display two alleles of the first intron, named by us 1R and 2R), fish #33 (found following sequencing also to possess allele...
Lyons PP, Turnbull JF, Dawson KA, Crumlish M (2015) Exploring the microbial diversity of the distal intestinal lumen and mucosa of farmed rainbow trout Oncorhynchus mykiss (Walbaum) using next generation sequencing (NGS). Aquacult Res 48(1):77–91 Article Google Scholar Merrifield DL, Dimitro...
This study investigated the effects of replacing fish meal (FM) with fermented soybean meal (FSM) on the growth performance, serum biochemical indices, intestinal health, microbial community and disease resistance of largemouth bass (Micropterus salmoides). The basal diet was designed to contain 350...
We thank the Broad Institute Genomics Platform for constructing and sequencing gar DNA and RNA libraries and J. Turner-Maier for the gar transcriptome assembly. We thank the teams of the Bayousphere Research Laboratory (Nicholls State University) and the University of Oregon Fish Facility for gar ...