研究人员还比较了 RAGE 和拉曼激活细胞弹射(RACE-seq)两种技术对 FISH-scRACS-seq 的适用性。结果显示,FISH-scRACS-seq 在所有组装指标(基因组完整性、测序偏差和组装连续性)上均优于 RACE-seq,能恢复更多基因 ,因此后续实验选择 RAGE 用于 FISH-scRACS-seq 。此外,通过将 FISH-scRACS-seq 获得的 SAG 与 s...
ARTseq-FISH reveals relative changes in both abundance and localisation of mRNAs and (phospho-)proteins during the first 48 hours of exit from pluripotency. We confirm these changes by conventional immunofluorescence and time-lapse microscopy. Chemical labelling, immunofluorescence, and single-cell ...
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PCR products were purified and pooled before sequencing on the MiSeq platform (Illumina). 4.7. Statistical Analysis Computations were performed using R version 3.2.3 (R Foundation for Statistical Computing, Vienna, Austria). Confidence intervals (CIs) for sensitivity and specificity were computed ...
RNA-seq Molecular Biology Gene Expression Antibody In Situ Hybridization Before you begin The protocol below describes the steps for simultaneously profiling of message ribonucleic acid (mRNAs), proteins, and phosphoproteins in individual mouse embryonic stem cells (mESCs). The method is antibody and ...