DNA binding assays established that MFP1 can discriminate between animal and plant MAR DNAs and non-MAR DNA fragments of similar size and AT content. Deletion mutants of MFP1 revealed a novel, discrete DNA binding domain near the C terminus of the protein. MFP1 is an in vitro substrate ...
Coiled-coil domains of intermediate filaments (IF) and prokaryotic IF-like proteins enable oligomerisation and filamentation, and no additional function is ascribed to these coiled-coil domains. However, an IF-like protein fromStreptomyces reticuliwas reported to display cellulose affinity. We demonstrat...
Protoplasts pellet was suspended in an equal volume of washing buffer and one drop of this suspension was placed onto each poly-L-lysine-coated grid. We used Penman's method for cell fractionation with some modifications. After plant cells were treated with CSK-Triton and RSB-Magik solution, a...
plant cells keratin-like protein intermediate filament-like system selective fractionation Western bⅠ. INTRODUCTION Intermediate filaments with a diameter of 10 nm, known as a cytoskeleton system, are extensively present in animal cells. Whether the intermediate filaments are present in plant cells remai...
The study of plant microtubules may provide clues. During growth of the cortical microtubule array in plant cells, microtubules align with each other in a self-reinforcing mechanism. When the plus end of a microtubule meets another microtubule at an angle of less than 40°, the first polymer’...
Whilst CAHS proteins are restricted to Eutardigrada97, the biological principle of coupling filamentous polymers with protein stabilisers to facilitate condensate formation is conserved across the plant and animal kingdoms14, 16, 43, 98, 99. This is no more so than in the eye lens, where ...