keratin-like proteinintermediate filament-like systemselective fractionationWestern blotⅠ. INTRODUCTION Intermediate filaments with a diameter of 10 nm, known as a cytoskeleton system, are extensively present in animal cells. Whether the intermediate filaments are present in plant cells remains to be a ...
Coiled-coil domains of intermediate filaments (IF) and prokaryotic IF-like proteins enable oligomerisation and filamentation, and no additional function is ascribed to these coiled-coil domains. However, an IF-like protein fromStreptomyces reticuliwas reported to display cellulose affinity. We demonstrat...
residues 102-107 do not form a helix, which would correspond to helix α4 in most other NTRs. This feature has been observed in other NTR-like proteins, however, in AlbA it likely plays a role in the interaction with AlbB. Sequence comparison of AlbA with other characterized NTR-like prot...
That IF system exists in plant protoplasts just like in animal cells, and their main component is keratin-like protein. Key words: keratin intermediate filament-like system, keratin-like protein, maize protoplasts. INTRODUCTION Since Ishikawa et al. (1968) found 10 nm filaments in animal cells,...
DNA binding assays established that MFP1 can discriminate between animal and plant MAR DNAs and non-MAR DNA fragments of similar size and AT content. Deletion mutants of MFP1 revealed a novel, discrete DNA binding domain near the C terminus of the protein. MFP1 is an in vitro substrate ...
stalk,stem- a slender or elongated structure that supports a plant or fungus or a plant part or plant organ 3.filament- a threadlike structure (as a chainlike series of cells) filum anatomical structure,bodily structure,body structure,complex body part,structure- a particular complex anatomical ...
1 Since the formal classification of the disease,2 a single gene has been identified as causative and encodes the Autoimmune Regulator (AIRE) protein.3 The AIRE protein contains several key domains, including two plant homeodomain (PHD) zinc fingers, four LXXLL motifs, a Sp100, AIRE-1, Nuc...
Three different classes of plant formins were described that contain e.g. an N-terminal transmembrane region, a PTEN-like domain that exhibits phosphatase activity or a RhoGAP domain that stimulates GTPase activity. Arabidopsis thaliana contains e.g. 21 different formins, while budding yeast ...
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Remarkably, this fusion protein, like All4981-GFP, formed cell-traversing filament-like or spindle-like strings in Anabaena (Supplementary Fig. 4G). In contrast, full length YFP-All4981 localized to the septa between two neighboring cells but also revealed indistinct cytosolic localization (...