Fluor- escent beads (488 nm fluorescent green) of 0.5 μm in size that are Streptavidin-tagged (BANGS LABORATORIES INC, https://www. bangslabs.com/order-form/CFDG003%7C15557) are mixed into the protein mixture (
bbln-1 is predicted to encode a single protein isoform of just 125 amino acids (aas) with a central coiled-coil domain ( aas 39–102) flanked by intrinsically disordered regions (Figure 1F). To investigate the localization pattern of BBLN-1, we engineered an endogenous BBLN-1::GFP fusion....